Electrochemical Sensing of Total Antioxidant Capacity and
Polyphenol Content in Wine Samples Using Amperometry
Online-Coupled with Microdialysis

J 2012

Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis

JAKUBEC, P; M BANCIROVA; V HALOUZKA; Antonín LOJEK; Milan ČÍŽ et al.

Základní údaje

Originální název

Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis

Autoři

JAKUBEC, P; M BANCIROVA; V HALOUZKA; Antonín LOJEK; Milan ČÍŽ; Petko DENEV; N CIBICEK; J VACEK; J VOSTALOVA; J ULRICHOVA a J HRBAC

Vydání

Journal of Agricultural and Food Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2012, 0021-8561

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.906

Označené pro přenos do RIV

DOI

https://doi.org/10.1021/jf3019886

UT WoS

000307487100010

Klíčová slova anglicky

wine; antioxidant capacity; phenolics; ORAC; TRAP; CL-TAC; amperometry; microdialysis

Změněno: 12. 9. 2017 12:27, doc. RNDr. Milan Číž, Ph.D.

Anotace

V originále

This work describes the method for total antioxidant capacity (TAC) and/or total content of phenolics (TCP) analysis in wines using microdialysis online-coupled with amperometric detection using a carbon microfiber working electrode. The system was tested on 10 selected wine samples, and the results were compared with total reactive antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), and chemiluminescent determination of total antioxidant capacity (CL-TAC) methods using Trolox and catechin as standards. Microdialysis online-coupled with amperometric detection gives similar results to the widely used cyclic voltammetry methodology and closely correlates with ORAC and TRAP. The problem of electrode fouling is overcome by the introduction of an electrochemical cleaning step (1-2 min at the potential of 0 V vs Ag/AgCl). Such a procedure is sufficient to fully regenerate the electrode response for both red and white wine samples as well as catechin/Trolox standards. The appropriate size of microdialysis probes enables easy automation of the electrochemical TAC/TCP measurement using 96-well microtitration plates.

Citovat

JAKUBEC, P; M BANCIROVA; V HALOUZKA; Antonín LOJEK; Milan ČÍŽ; Petko DENEV; N CIBICEK; J VACEK; J VOSTALOVA; J ULRICHOVA a J HRBAC. Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis. Journal of Agricultural and Food Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2012, roč. 60, č. 32, s. 7836-7843. ISSN 0021-8561. Dostupné z: https://doi.org/10.1021/jf3019886.

@article{1389162,
   author = {Jakubec, P and Bancirova, M and Halouzka, V and Lojek, Antonín and Číž, Milan and Denev, Petko and Cibicek, N and Vacek, J and Vostalova, J and Ulrichova, J and Hrbac, J},
   article_location = {WASHINGTON},
   article_number = {32},
   doi = {https://doi.org/10.1021/jf3019886},
   keywords = {wine; antioxidant capacity; phenolics; ORAC; TRAP; CL-TAC; amperometry; microdialysis},
   language = {eng},
   issn = {0021-8561},
   journal = {Journal of Agricultural and Food Chemistry},
   title = {Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis},
   volume = {60},
   year = {2012}
}

TY  - JOUR
ID  - 1389162
AU  - Jakubec, P - Bancirova, M - Halouzka, V - Lojek, Antonín - Číž, Milan - Denev, Petko - Cibicek, N - Vacek, J - Vostalova, J - Ulrichova, J - Hrbac, J
PY  - 2012
TI  - Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis
JF  - Journal of Agricultural and Food Chemistry
VL  - 60
IS  - 32
SP  - 7836-7843
EP  - 7836-7843
PB  - AMER CHEMICAL SOC
SN  - 00218561
KW  - wine
KW  - antioxidant capacity
KW  - phenolics
KW  - ORAC
KW  - TRAP
KW  - CL-TAC
KW  - amperometry
KW  - microdialysis
N2  - This work describes the method for total antioxidant capacity (TAC) and/or total content of phenolics (TCP) analysis in wines using microdialysis online-coupled with amperometric detection using a carbon microfiber working electrode. The system was tested on 10 selected wine samples, and the results were compared with total reactive antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), and chemiluminescent determination of total antioxidant capacity (CL-TAC) methods using Trolox and catechin as standards. Microdialysis online-coupled with amperometric detection gives similar results to the widely used cyclic voltammetry methodology and closely correlates with ORAC and TRAP. The problem of electrode fouling is overcome by the introduction of an electrochemical cleaning step (1-2 min at the potential of 0 V vs Ag/AgCl). Such a procedure is sufficient to fully regenerate the electrode response for both red and white wine samples as well as catechin/Trolox standards. The appropriate size of microdialysis probes enables easy automation of the electrochemical TAC/TCP measurement using 96-well microtitration plates.
ER  -

JAKUBEC, P; M BANCIROVA; V HALOUZKA; Antonín LOJEK; Milan ČÍŽ; Petko DENEV; N CIBICEK; J VACEK; J VOSTALOVA; J ULRICHOVA a J HRBAC. Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis. \textit{Journal of Agricultural and Food Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2012, roč.~60, č.~32, s.~7836-7843. ISSN~0021-8561. Dostupné z: https://doi.org/10.1021/jf3019886.

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