Serotonin and its 5-HT2 receptor agonist DOI hydrochloride
inhibit the oxidative burst in total leukocytes but not in
isolated neutrophils

J 2010

Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils

PRACHAŘOVÁ, Lucie; Kateřina OKÉNKOVÁ; Antonín LOJEK a Milan ČÍŽ

Základní údaje

Originální název

Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils

Autoři

PRACHAŘOVÁ, Lucie; Kateřina OKÉNKOVÁ; Antonín LOJEK a Milan ČÍŽ

Vydání

Life Sciences, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2010, 0024-3205

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.451

Označené pro přenos do RIV

DOI

https://doi.org/10.1016/j.lfs.2010.02.003

UT WoS

000276142300009

Klíčová slova anglicky

Chemiluminescence; Leukocyte; Neutrophil; Oxidative burst; Serotonin; Serotonin receptor

Změněno: 12. 9. 2017 13:26, doc. RNDr. Milan Číž, Ph.D.

Anotace

V originále

Aims Serotonin (5-HT) is capable of reducing the oxidative burst of professional phagocytes In this study, we investigated whether 5-HT mediates this modulation via 5-HT receptors (5-HTR) or whether this is due instead to 5-HT antioxidative properties Main methods The leukocytes or polymorphonuclear leukocytes (PMNL) were isolated from human blood, and their ability to produce reactive oxygen species (ROS) after 5-HT or its agonist treatment was tested by lummol-enhanced chemiluminescence (CL) analysis Key findings It was found that 5-HTR2 agonist DOI hydrochloride does not have any antioxidative properties, despite its ability to inhibit the CL response of activated human total leukocytes On the other hand, DOI hydrochloride was unable to inhibit the CL response of activated human PMNL It seems that the reduction of the oxidative burst of professional phagocytes was evoked by the activation of 5-HTR not on the neutrophil surface but on the surface of different leukocytes, which produced anti-inflammatory cytokines with NADPH oxidase activity modulating properties Significance Platelets and activated PMNL are in tight contact at sites of inflammation 5-HT released from platelets might have a protective function against PMNL-derived oxidative stress and oxidative damages (C) 2010 Elsevier Inc All rights reserved

Citovat

PRACHAŘOVÁ, Lucie; Kateřina OKÉNKOVÁ; Antonín LOJEK a Milan ČÍŽ. Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils. Life Sciences. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2010, roč. 86, 13-14, s. 518-523. ISSN 0024-3205. Dostupné z: https://doi.org/10.1016/j.lfs.2010.02.003.

@article{1389167,
   author = {Prachařová, Lucie and Okénková, Kateřina and Lojek, Antonín and Číž, Milan},
   article_location = {OXFORD},
   article_number = {13-14},
   doi = {https://doi.org/10.1016/j.lfs.2010.02.003},
   keywords = {Chemiluminescence; Leukocyte; Neutrophil; Oxidative burst; Serotonin; Serotonin receptor},
   language = {eng},
   issn = {0024-3205},
   journal = {Life Sciences},
   title = {Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils},
   volume = {86},
   year = {2010}
}

TY  - JOUR
ID  - 1389167
AU  - Prachařová, Lucie - Okénková, Kateřina - Lojek, Antonín - Číž, Milan
PY  - 2010
TI  - Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils
JF  - Life Sciences
VL  - 86
IS  - 13-14
SP  - 518-523
EP  - 518-523
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00243205
KW  - Chemiluminescence
KW  - Leukocyte
KW  - Neutrophil
KW  - Oxidative burst
KW  - Serotonin
KW  - Serotonin receptor
N2  - Aims Serotonin (5-HT) is capable of reducing the oxidative burst of professional phagocytes In this study, we investigated whether 5-HT mediates this modulation via 5-HT receptors (5-HTR) or whether this is due instead to 5-HT antioxidative properties Main methods The leukocytes or polymorphonuclear leukocytes (PMNL) were isolated from human blood, and their ability to produce reactive oxygen species (ROS) after 5-HT or its agonist treatment was tested by lummol-enhanced chemiluminescence (CL) analysis Key findings It was found that 5-HTR2 agonist DOI hydrochloride does not have any antioxidative properties, despite its ability to inhibit the CL response of activated human total leukocytes On the other hand, DOI hydrochloride was unable to inhibit the CL response of activated human PMNL It seems that the reduction of the oxidative burst of professional phagocytes was evoked by the activation of 5-HTR not on the neutrophil surface but on the surface of different leukocytes, which produced anti-inflammatory cytokines with NADPH oxidase activity modulating properties Significance Platelets and activated PMNL are in tight contact at sites of inflammation 5-HT released from platelets might have a protective function against PMNL-derived oxidative stress and oxidative damages (C) 2010 Elsevier Inc All rights reserved
ER  -

PRACHAŘOVÁ, Lucie; Kateřina OKÉNKOVÁ; Antonín LOJEK a Milan ČÍŽ. Serotonin and its 5-HT2 receptor agonist DOI hydrochloride inhibit the oxidative burst in total leukocytes but not in isolated neutrophils. \textit{Life Sciences}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2010, roč.~86, 13-14, s.~518-523. ISSN~0024-3205. Dostupné z: https://doi.org/10.1016/j.lfs.2010.02.003.

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