Growth suppression of human breast carcinoma stem cells by
lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl
radical-modified collagen

J 2010

Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen

CIPAK, A; L MRAKOVCIC; Milan ČÍŽ; Antonín LOJEK; B MIHAYLOVA et al.

Základní údaje

Originální název

Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen

Autoři

CIPAK, A; L MRAKOVCIC; Milan ČÍŽ; Antonín LOJEK; B MIHAYLOVA; I GOSHEV; M JAGANJAC; M CINDRIC; S SITIC; M MARGARITONI; G WAEG; M BALIC a N ZARKOVIC

Vydání

Acta Biochimica Polonica, WARSAW, Polish Academy of Sciences, Committee of Biochemistry, 2010, 0001-527X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.234

Označené pro přenos do RIV

UT WoS

000279416900006

Klíčová slova anglicky

SUM159; breast cancer stem cells; 4-hydroxynonenal; extracellular matrix; collagen; oxidative homeostasis

Změněno: 12. 9. 2017 12:40, doc. RNDr. Milan Číž, Ph.D.

Anotace

V originále

Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44(+)CD24(-/low)ESA(+) usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44(+)CD24(-/low)ESA(+) cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 mu M) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.

Citovat

CIPAK, A; L MRAKOVCIC; Milan ČÍŽ; Antonín LOJEK; B MIHAYLOVA; I GOSHEV; M JAGANJAC; M CINDRIC; S SITIC; M MARGARITONI; G WAEG; M BALIC a N ZARKOVIC. Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen. Acta Biochimica Polonica. WARSAW: Polish Academy of Sciences, Committee of Biochemistry, 2010, roč. 57, č. 2, s. 165-171. ISSN 0001-527X.

@article{1389170,
   author = {Cipak, A and Mrakovcic, L and Číž, Milan and Lojek, Antonín and Mihaylova, B and Goshev, I and Jaganjac, M and Cindric, M and Sitic, S and Margaritoni, M and Waeg, G and Balic, M and Zarkovic, N},
   article_location = {WARSAW},
   article_number = {2},
   keywords = {SUM159; breast cancer stem cells; 4-hydroxynonenal; extracellular matrix; collagen; oxidative homeostasis},
   language = {eng},
   issn = {0001-527X},
   journal = {Acta Biochimica Polonica},
   title = {Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen},
   volume = {57},
   year = {2010}
}

TY  - JOUR
ID  - 1389170
AU  - Cipak, A - Mrakovcic, L - Číž, Milan - Lojek, Antonín - Mihaylova, B - Goshev, I - Jaganjac, M - Cindric, M - Sitic, S - Margaritoni, M - Waeg, G - Balic, M - Zarkovic, N
PY  - 2010
TI  - Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen
JF  - Acta Biochimica Polonica
VL  - 57
IS  - 2
SP  - 165-171
EP  - 165-171
PB  - Polish Academy of Sciences, Committee of Biochemistry
SN  - 0001527X
KW  - SUM159
KW  - breast cancer stem cells
KW  - 4-hydroxynonenal
KW  - extracellular matrix
KW  - collagen
KW  - oxidative homeostasis
N2  - Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44(+)CD24(-/low)ESA(+) usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44(+)CD24(-/low)ESA(+) cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 mu M) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.
ER  -

CIPAK, A; L MRAKOVCIC; Milan ČÍŽ; Antonín LOJEK; B MIHAYLOVA; I GOSHEV; M JAGANJAC; M CINDRIC; S SITIC; M MARGARITONI; G WAEG; M BALIC a N ZARKOVIC. Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen. \textit{Acta Biochimica Polonica}. WARSAW: Polish Academy of Sciences, Committee of Biochemistry, 2010, roč.~57, č.~2, s.~165-171. ISSN~0001-527X.

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