Interactions of oxidatively modified calf skin collagen with
platelets and phagocytes

J 2009

Interactions of oxidatively modified calf skin collagen with platelets and phagocytes

ČÍŽ, Milan; H CIZOVA; K PEJCHALOVA; V JANCINOVA; I GOSHEV et al.

Základní údaje

Originální název

Interactions of oxidatively modified calf skin collagen with platelets and phagocytes

Autoři

ČÍŽ, Milan; H CIZOVA; K PEJCHALOVA; V JANCINOVA; I GOSHEV; B MIHAYLOVA; R NOSAL a A LOJEK

Vydání

Neuroendocrinology Letters, STOCKHOLM, MAGHIRA & MAAS PUBLICATIONS, 2009, 0172-780X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.047

Označené pro přenos do RIV

UT WoS

000275266600025

Klíčová slova anglicky

collagen; extracellular matrix; oxidative burst; oxidative stress; phagocytes; platelet aggregation

Změněno: 12. 9. 2017 13:28, doc. RNDr. Milan Číž, Ph.D.

Anotace

V originále

OBJECTIVES: The effects of non-modified and oxidatively modified calf skin collagen type I on platelet aggregation and the oxidative burst of phagocytes were examined in the framework of a general hypothesis that collagen, platelets and phagocytes cooperate to modulate the oxidative burst of phagocytes and the extent of oxidative stress. MATERIALS AND METHODS: Calf skin collagen type I was subjected to oxidative modification by hydrogen peroxide or hydroxyl radical. Thermal denaturation of collagen was performed in a spectrophotometer equipped with a temperature gradient device. The aggregation of isolated human platelets obtained after differential centrifugation was measured using a dual-channel aggregometer. The production of reactive oxygen species by human whole blood phagocytes was evaluated by luminol-enhanced chemiluminescence. RESULTS: Oxidative modification of collagen samples was characterized by a decrease in denaturation transition temperature. Oxidatively modified samples showed a modified SDS-PAGE pattern, evidencing a significant destruction of the collagen. All oxidatively modified collagen samples, independent of the oxidation treatment applied, lost their platelet-aggregating and phagocyte oxidative burst-inducing activity. CONCLUSION: The results suggest that reactive oxygen species were able to modify collagen. On the other hand, oxidatively modified collagen lost its activating properties towards platelets and phagocytes.

Citovat

ČÍŽ, Milan; H CIZOVA; K PEJCHALOVA; V JANCINOVA; I GOSHEV; B MIHAYLOVA; R NOSAL a A LOJEK. Interactions of oxidatively modified calf skin collagen with platelets and phagocytes. Neuroendocrinology Letters. STOCKHOLM: MAGHIRA & MAAS PUBLICATIONS, 2009, roč. 30, s. 128-132. ISSN 0172-780X.

@article{1389172,
   author = {Číž, Milan and Cizova, H and Pejchalova, K and Jancinova, V and Goshev, I and Mihaylova, B and Nosal, R and Lojek, A},
   article_location = {STOCKHOLM},
   keywords = {collagen; extracellular matrix; oxidative burst; oxidative stress; phagocytes; platelet aggregation},
   language = {eng},
   issn = {0172-780X},
   journal = {Neuroendocrinology Letters},
   title = {Interactions of oxidatively modified calf skin collagen with platelets and phagocytes},
   volume = {30},
   year = {2009}
}

TY  - JOUR
ID  - 1389172
AU  - Číž, Milan - Cizova, H - Pejchalova, K - Jancinova, V - Goshev, I - Mihaylova, B - Nosal, R - Lojek, A
PY  - 2009
TI  - Interactions of oxidatively modified calf skin collagen with platelets and phagocytes
JF  - Neuroendocrinology Letters
VL  - 30
SP  - 128-132
EP  - 128-132
PB  - MAGHIRA & MAAS PUBLICATIONS
SN  - 0172780X
KW  - collagen
KW  - extracellular matrix
KW  - oxidative burst
KW  - oxidative stress
KW  - phagocytes
KW  - platelet aggregation
N2  - OBJECTIVES: The effects of non-modified and oxidatively modified calf skin collagen type I on platelet aggregation and the oxidative burst of phagocytes were examined in the framework of a general hypothesis that collagen, platelets and phagocytes cooperate to modulate the oxidative burst of phagocytes and the extent of oxidative stress. MATERIALS AND METHODS: Calf skin collagen type I was subjected to oxidative modification by hydrogen peroxide or hydroxyl radical. Thermal denaturation of collagen was performed in a spectrophotometer equipped with a temperature gradient device. The aggregation of isolated human platelets obtained after differential centrifugation was measured using a dual-channel aggregometer. The production of reactive oxygen species by human whole blood phagocytes was evaluated by luminol-enhanced chemiluminescence. RESULTS: Oxidative modification of collagen samples was characterized by a decrease in denaturation transition temperature. Oxidatively modified samples showed a modified SDS-PAGE pattern, evidencing a significant destruction of the collagen. All oxidatively modified collagen samples, independent of the oxidation treatment applied, lost their platelet-aggregating and phagocyte oxidative burst-inducing activity. CONCLUSION: The results suggest that reactive oxygen species were able to modify collagen. On the other hand, oxidatively modified collagen lost its activating properties towards platelets and phagocytes.
ER  -

ČÍŽ, Milan; H CIZOVA; K PEJCHALOVA; V JANCINOVA; I GOSHEV; B MIHAYLOVA; R NOSAL a A LOJEK. Interactions of oxidatively modified calf skin collagen with platelets and phagocytes. \textit{Neuroendocrinology Letters}. STOCKHOLM: MAGHIRA \&{}amp; MAAS PUBLICATIONS, 2009, roč.~30, s.~128-132. ISSN~0172-780X.

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