Oncolysate-loaded Escherichia coli bacterial ghosts enhance the
stimulatory capacity of human dendritic cells

J 2017

Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells

MICHÁLEK, Jaroslav; Renata HÉŽOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; J. GABKOVA; M. STRIOGA et al.

Základní údaje

Originální název

Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells

Autoři

MICHÁLEK, Jaroslav; Renata HÉŽOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; J. GABKOVA; M. STRIOGA; W. LUBITZ a P. KUDELA

Vydání

CANCER IMMUNOLOGY IMMUNOTHERAPY, NEW YORK, SPRINGER, 2017, 0340-7004

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.225

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00097523

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Bacterial ghosts; Dendritic cells; T cell stimulation; Cancer immunotherapy; Natural adjuvant; Vaccine

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2018 14:30, Soňa Böhmová

Anotace

V originále

The natural adjuvant properties of bacterial ghosts (BGs) lie within the presence of intact pathogen-associated molecular patterns on their surface. BGs can improve the direct delivery, natural processing and presentation of target antigens within dendritic cells (DCs). Moreover, sensitization of human DCs by cancer cell lysate (oncolysate)-loaded BGs in the presence of IFN-alpha and GM-CSF enhanced DC maturation as indicated by an increased expression of maturation markers and costimulatory molecules, higher production of IL-12p70 and stimulation of significantly increased proliferation of both autologous CD4(+) and CD8(+) T cells compared to DCs matured in the presence of purified lipopolysaccharide. The induced T cells efficiently recognized oncolysate-derived tumor-associated antigens expressed by cancer cells used for the production of oncolysate. Our optimized one-step simultaneous antigen delivery and DC maturation-inducing method emerges as a promising tool for the development and implementation of next-generation cellular cancer immunotherapies.

Citovat

MICHÁLEK, Jaroslav; Renata HÉŽOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; J. GABKOVA; M. STRIOGA; W. LUBITZ a P. KUDELA. Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells. CANCER IMMUNOLOGY IMMUNOTHERAPY. NEW YORK: SPRINGER, 2017, roč. 66, č. 2, s. 149-159. ISSN 0340-7004. Dostupné z: https://doi.org/10.1007/s00262-016-1932-4.

@article{1389204,
   author = {Michálek, Jaroslav and Héžová, Renata and Turánek Knötigová, Pavlína and Gabkova, J. and Strioga, M. and Lubitz, W. and Kudela, P.},
   article_location = {NEW YORK},
   article_number = {2},
   doi = {https://doi.org/10.1007/s00262-016-1932-4},
   keywords = {Bacterial ghosts; Dendritic cells; T cell stimulation; Cancer immunotherapy; Natural adjuvant; Vaccine},
   language = {eng},
   issn = {0340-7004},
   journal = {CANCER IMMUNOLOGY IMMUNOTHERAPY},
   title = {Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells},
   volume = {66},
   year = {2017}
}

TY  - JOUR
ID  - 1389204
AU  - Michálek, Jaroslav - Héžová, Renata - Turánek Knötigová, Pavlína - Gabkova, J. - Strioga, M. - Lubitz, W. - Kudela, P.
PY  - 2017
TI  - Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells
JF  - CANCER IMMUNOLOGY IMMUNOTHERAPY
VL  - 66
IS  - 2
SP  - 149-159
EP  - 149-159
PB  - SPRINGER
SN  - 03407004
KW  - Bacterial ghosts
KW  - Dendritic cells
KW  - T cell stimulation
KW  - Cancer immunotherapy
KW  - Natural adjuvant
KW  - Vaccine
N2  - The natural adjuvant properties of bacterial ghosts (BGs) lie within the presence of intact pathogen-associated molecular patterns on their surface. BGs can improve the direct delivery, natural processing and presentation of target antigens within dendritic cells (DCs). Moreover, sensitization of human DCs by cancer cell lysate (oncolysate)-loaded BGs in the presence of IFN-alpha and GM-CSF enhanced DC maturation as indicated by an increased expression of maturation markers and costimulatory molecules, higher production of IL-12p70 and stimulation of significantly increased proliferation of both autologous CD4(+) and CD8(+) T cells compared to DCs matured in the presence of purified lipopolysaccharide. The induced T cells efficiently recognized oncolysate-derived tumor-associated antigens expressed by cancer cells used for the production of oncolysate. Our optimized one-step simultaneous antigen delivery and DC maturation-inducing method emerges as a promising tool for the development and implementation of next-generation cellular cancer immunotherapies.
ER  -

MICHÁLEK, Jaroslav; Renata HÉŽOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; J. GABKOVA; M. STRIOGA; W. LUBITZ a P. KUDELA. Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells. \textit{CANCER IMMUNOLOGY IMMUNOTHERAPY}. NEW YORK: SPRINGER, 2017, roč.~66, č.~2, s.~149-159. ISSN~0340-7004. Dostupné z: https://doi.org/10.1007/s00262-016-1932-4.

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