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@article{1389397,
author = {Nyc, O. and Tejkalová, Renata and Kříž, Zdeněk and Růžička, Filip and Kubíček, Luboš and Matejkova, J. and Kuijper, E. and Krutova, M.},
article_location = {New Rochelle},
article_number = {5},
doi = {http://dx.doi.org/10.1089/mdr.2016.0159},
keywords = {Clostridium difficile; PCR ribotype 001; capillary electrophoresis ribotyping; MLVA; antimicrobial drug resistance; Thr82Ile},
language = {eng},
issn = {1076-6294},
journal = {Microbial Drug Resistance},
title = {Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis},
volume = {23},
year = {2017}
}
TY - JOUR
ID - 1389397
AU - Nyc, O. - Tejkalová, Renata - Kříž, Zdeněk - Růžička, Filip - Kubíček, Luboš - Matejkova, J. - Kuijper, E. - Krutova, M.
PY - 2017
TI - Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis
JF - Microbial Drug Resistance
VL - 23
IS - 5
SP - 609-615
EP - 609-615
PB - Mary Ann Liebert
SN - 10766294
KW - Clostridium difficile
KW - PCR ribotype 001
KW - capillary electrophoresis ribotyping
KW - MLVA
KW - antimicrobial drug resistance
KW - Thr82Ile
N2 - Aim: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies. Methods: Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested. Findings: Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC 32mg/L), moxifloxacin (MIC 32mg/L), and clindamycin (MIC 256mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative. Conclusion: Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
ER -
NYC, O., Renata TEJKALOVÁ, Zdeněk KŘÍŽ, Filip RŮŽIČKA, Luboš KUBÍČEK, J. MATEJKOVA, E. KUIJPER a M. KRUTOVA. Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis. \textit{Microbial Drug Resistance}. New Rochelle: Mary Ann Liebert, 2017, roč.~23, č.~5, s.~609-615. ISSN~1076-6294. Dostupné z: https://dx.doi.org/10.1089/mdr.2016.0159.
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