Rapid single-step upconversion-linked immunosorbent assay for
diclofenac

J 2017

Rapid single-step upconversion-linked immunosorbent assay for diclofenac

HLAVÁČEK, Antonín, Miroslav PETEREK, Zdeněk FARKA, Matthias J. MICKERT, Leonhard PRECHTL et. al.

Základní údaje

Originální název

Rapid single-step upconversion-linked immunosorbent assay for diclofenac

Autoři

HLAVÁČEK, Antonín (203 Česká republika, domácí), Miroslav PETEREK (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), Matthias J. MICKERT (276 Německo), Leonhard PRECHTL (276 Německo), Dietmar KNOPP (276 Německo) a Hans H. GORRIS (276 Německo, garant)

Vydání

Microchimica Acta, Vienna, Springer, 2017, 0026-3672

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.705

Kód RIV

RIV/00216224:14740/17:00097559

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00604-017-2456-0

UT WoS

000410517400062

Klíčová slova anglicky

Bioconjugation; Electrophoretic purification; Immunoassay; Luminescence; Pharmaceutical micropollutant; Photon-upconversion

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 11:03, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The non-steroidal anti-inflammatory drug and analgesic diclofenac is a common micropollutant in water. A direct competitive upconversion-linked immunosorbent assay (ULISA) for diclofenac has been developed that is based on a nanoparticulate tracer consisting of a NaYF4:Yb,Er core (diameter of ~90 nm, excitation and emission wavelenghts 980 and 535 nm, respectively) enclosed by a carboxylated silica shell and finally coated with diclofenac-conjugated bovine gamma-globulin. The proteinaceous coating prevents non-specific adsorption of the tracer to the microtiter plate and provides a structurally flexible linker for surface-exposed diclofenac to warrant efficient competition with free (analyte) diclofenac in real water samples. The tracer was purified by gel electrophoresis and lyophilized. Both processes have no adverse effects on the immunoassay. All assay components can be stored in a dry state without a cooling chain, and can be reactivated on demand. Hence, this ULISA is well suited for environmental monitoring in low-resource settings. The ULISA has a similar limit of detection (20 pg mL-1; equivalent to 70 pM) as the conventional ELISA, but the time for analysis is reduced to 70 min because no enzymatic amplification steps are involved.

Návaznosti

LD15023, projekt VaV

Název: Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Příprava diskrétních biokonjugátů photon-upconversion nanočástic

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

HLAVÁČEK, Antonín, Miroslav PETEREK, Zdeněk FARKA, Matthias J. MICKERT, Leonhard PRECHTL, Dietmar KNOPP a Hans H. GORRIS. Rapid single-step upconversion-linked immunosorbent assay for diclofenac. Microchimica Acta. Vienna: Springer, 2017, roč. 184, č. 10, s. 4159-4165. ISSN 0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-017-2456-0.

@article{1389407,
   author = {Hlaváček, Antonín and Peterek, Miroslav and Farka, Zdeněk and Mickert, Matthias J. and Prechtl, Leonhard and Knopp, Dietmar and Gorris, Hans H.},
   article_location = {Vienna},
   article_number = {10},
   doi = {http://dx.doi.org/10.1007/s00604-017-2456-0},
   keywords = {Bioconjugation; Electrophoretic purification; Immunoassay; Luminescence; Pharmaceutical micropollutant; Photon-upconversion},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchimica Acta},
   title = {Rapid single-step upconversion-linked immunosorbent assay for diclofenac},
   url = {https://link.springer.com/article/10.1007%2Fs00604-017-2456-0},
   volume = {184},
   year = {2017}
}

TY  - JOUR
ID  - 1389407
AU  - Hlaváček, Antonín - Peterek, Miroslav - Farka, Zdeněk - Mickert, Matthias J. - Prechtl, Leonhard - Knopp, Dietmar - Gorris, Hans H.
PY  - 2017
TI  - Rapid single-step upconversion-linked immunosorbent assay for diclofenac
JF  - Microchimica Acta
VL  - 184
IS  - 10
SP  - 4159-4165
EP  - 4159-4165
PB  - Springer
SN  - 00263672
KW  - Bioconjugation
KW  - Electrophoretic purification
KW  - Immunoassay
KW  - Luminescence
KW  - Pharmaceutical micropollutant
KW  - Photon-upconversion
UR  - https://link.springer.com/article/10.1007%2Fs00604-017-2456-0
L2  - https://link.springer.com/article/10.1007%2Fs00604-017-2456-0
N2  - The non-steroidal anti-inflammatory drug and analgesic diclofenac is a common micropollutant in water. A direct competitive upconversion-linked immunosorbent assay (ULISA) for diclofenac has been developed that is based on a nanoparticulate tracer consisting of a NaYF4:Yb,Er core (diameter of ~90 nm, excitation and emission wavelenghts 980 and 535 nm, respectively) enclosed by a carboxylated silica shell and finally coated with diclofenac-conjugated bovine gamma-globulin. The proteinaceous coating prevents non-specific adsorption of the tracer to the microtiter plate and provides a structurally flexible linker for surface-exposed diclofenac to warrant efficient competition with free (analyte) diclofenac in real water samples. The tracer was purified by gel electrophoresis and lyophilized. Both processes have no adverse effects on the immunoassay. All assay components can be stored in a dry state without a cooling chain, and can be reactivated on demand. Hence, this ULISA is well suited for environmental monitoring in low-resource settings. The ULISA has a similar limit of detection (20 pg mL-1; equivalent to 70 pM) as the conventional ELISA, but the time for analysis is reduced to 70 min because no enzymatic amplification steps are involved.
ER  -

HLAVÁČEK, Antonín, Miroslav PETEREK, Zdeněk FARKA, Matthias J. MICKERT, Leonhard PRECHTL, Dietmar KNOPP a Hans H. GORRIS. Rapid single-step upconversion-linked immunosorbent assay for diclofenac. \textit{Microchimica Acta}. Vienna: Springer, 2017, roč.~184, č.~10, s.~4159-4165. ISSN~0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-017-2456-0.

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