Deregulated expression of long non-coding RNA UCA1 in multiple
myeloma

J 2017

Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

SEDLAŘÍKOVÁ, Lenka; Barbora GROMESOVÁ; Veronika KUBACZKOVÁ; Lenka RADOVÁ; Jana FILIPOVA et al.

Základní údaje

Originální název

Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

Autoři

Vydání

European Journal of Haematology, Hoboken, Wiley-Blackwell, 2017, 0902-4441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.595

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00095660

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

biomarker; long non-coding RNA; multiple myeloma; plasma cells; prognosis; quantitative real-time PCR

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 14:10, Soňa Böhmová

Anotace

V originále

ObjectivesLong non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. MethodsExpression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. ResultsOur analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. ConclusionOur study suggests a possible prognostic impact of UCA1 expression levels on MM patients.

Návaznosti

NV15-29508A, projekt VaV

Název: Cirkulující nukleové kyseliny jako markery progrese mnohočetného myelomu

Citovat

SEDLAŘÍKOVÁ, Lenka; Barbora GROMESOVÁ; Veronika KUBACZKOVÁ; Lenka RADOVÁ; Jana FILIPOVA; Jiří JARKOVSKÝ; Lucie BROŽOVÁ; Roberta VELICHOVÁ; Martina ALMÁŠI; Miroslav PENKA; Renata BEZDĚKOVÁ; Martin ŠTORK; Zdeněk ADAM; Luděk POUR; Marta KREJČÍ; Petr KUGLÍK; Roman HÁJEK a Sabina ŠEVČÍKOVÁ. Deregulated expression of long non-coding RNA UCA1 in multiple myeloma. European Journal of Haematology. Hoboken: Wiley-Blackwell, 2017, roč. 99, č. 3, s. 223-233. ISSN 0902-4441. Dostupné z: https://doi.org/10.1111/ejh.12908.

@article{1389453,
   author = {Sedlaříková, Lenka and Gromesová, Barbora and Kubaczková, Veronika and Radová, Lenka and Filipova, Jana and Jarkovský, Jiří and Brožová, Lucie and Velichová, Roberta and Almáši, Martina and Penka, Miroslav and Bezděková, Renata and Štork, Martin and Adam, Zdeněk and Pour, Luděk and Krejčí, Marta and Kuglík, Petr and Hájek, Roman and Ševčíková, Sabina},
   article_location = {Hoboken},
   article_number = {3},
   doi = {https://doi.org/10.1111/ejh.12908},
   keywords = {biomarker; long non-coding RNA; multiple myeloma; plasma cells; prognosis; quantitative real-time PCR},
   language = {eng},
   issn = {0902-4441},
   journal = {European Journal of Haematology},
   title = {Deregulated expression of long non-coding RNA UCA1 in multiple myeloma},
   volume = {99},
   year = {2017}
}

TY  - JOUR
ID  - 1389453
AU  - Sedlaříková, Lenka - Gromesová, Barbora - Kubaczková, Veronika - Radová, Lenka - Filipova, Jana - Jarkovský, Jiří - Brožová, Lucie - Velichová, Roberta - Almáši, Martina - Penka, Miroslav - Bezděková, Renata - Štork, Martin - Adam, Zdeněk - Pour, Luděk - Krejčí, Marta - Kuglík, Petr - Hájek, Roman - Ševčíková, Sabina
PY  - 2017
TI  - Deregulated expression of long non-coding RNA UCA1 in multiple myeloma
JF  - European Journal of Haematology
VL  - 99
IS  - 3
SP  - 223-233
EP  - 223-233
PB  - Wiley-Blackwell
SN  - 09024441
KW  - biomarker
KW  - long non-coding RNA
KW  - multiple myeloma
KW  - plasma cells
KW  - prognosis
KW  - quantitative real-time PCR
N2  - ObjectivesLong non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. MethodsExpression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. ResultsOur analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. ConclusionOur study suggests a possible prognostic impact of UCA1 expression levels on MM patients.
ER  -

SEDLAŘÍKOVÁ, Lenka; Barbora GROMESOVÁ; Veronika KUBACZKOVÁ; Lenka RADOVÁ; Jana FILIPOVA; Jiří JARKOVSKÝ; Lucie BROŽOVÁ; Roberta VELICHOVÁ; Martina ALMÁŠI; Miroslav PENKA; Renata BEZDĚKOVÁ; Martin ŠTORK; Zdeněk ADAM; Luděk POUR; Marta KREJČÍ; Petr KUGLÍK; Roman HÁJEK a Sabina ŠEVČÍKOVÁ. Deregulated expression of long non-coding RNA UCA1 in multiple myeloma. \textit{European Journal of Haematology}. Hoboken: Wiley-Blackwell, 2017, roč.~99, č.~3, s.~223-233. ISSN~0902-4441. Dostupné z: https://doi.org/10.1111/ejh.12908.

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