Lipopolysaccharide treatment of schwannoma cells induced acute
elevation of inflammatory mediators

a 2017

Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators

KOHOUTKOVÁ, Marcela; Andrea KORIMOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators

Autoři

KOHOUTKOVÁ, Marcela; Andrea KORIMOVÁ a Petr DUBOVÝ

Vydání

Morphology 2017 50th International Congress of the Czech Anatomical Society 54th Lojda Symposium in Histochemistry, 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30103 Neurosciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00100720

Organizační jednotka

Lékařská fakulta

ISBN

978-80-263-1315-1

Klíčová slova česky

Lipopolysaccharid; Schwannova buňka; neurozánět

Klíčová slova anglicky

Lipopolysaccharide; Schwann cell; neuroinflammation

Změněno: 5. 12. 2018 10:12, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators Kohoutková M. (1), Korimová A. (1), Dubový P. (1) (1) Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic Wallerian degeneration is a process of stereotypical reactions of Schwann cells which is important prerequisite for reparation of the damage nerve. Inflammatory activation of Schwann cells can be triggered by endogenous ligands produced during Wallerian degeneration or exogenous pathological molecules via Toll-like receptors (TLRs) [1,2]. Lipopolysaccharide (LPS) was used as a prototypical ligand for activation intracellular immune response of schwannoma cells. Acute inflammatory response of schwannoma cells is associated with increased production of cytokines and transcription factors [3]. We studied proteins the most often associated with inflammation – STAT3 and IL-6 and receptor TLR4, which can transduce inflammatory signal to cell. Rat schwannoma cells (RT4-D6P2T) were treated with LPS (10ng/ml) for 1 hour to induce acute inflammatory responses. TLR4, pSTAT3 and IL-6 proteins were studied by immunocytochemistry and Western blot analysis. We observed dynamic changes of TLR4 deposits in the cell cytoplasm but not in the cell surface. LPS treatment induced enlarging of early endosomes in schwannoma cells. We also found significantly increased levels of pSTAT3 and IL-6 proteins after LPS treatment indicating their crucial role in the induction of inflammation cascade in Schwann cells. [1] S. Rotshenker, Wallerian degeneration: the innate-immune response to traumatic nerve injury., J. Neuroinflammation. 8 (2011) 109. [2] E. Ydens, G. Lornet, V. Smits, S. Goethals, V. Timmerman, S. Janssens, The neuroinflammatory role of Schwann cells in disease, Neurobiol. Dis. 55 (2013) 95–103. [3] H.N. Hao, J.D. Peduzzi-Nelson, P.J. VandeVord, K. Barami, S.P. DeSilva, D. Pelinkovic, L.G. Morawa, Lipopolysaccharide-induced inflammatory cytokine production by Schwann’s cells dependent upon TLR4 expression, J. Neuroimmunol. 212 (2009) 26– 34. Supported by grant 16-08508S of The Czech Science Foundation.

Návaznosti

GA16-08508S, projekt VaV

Název: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Akronym: ERP)

Investor: Grantová agentura ČR, Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny

Citovat

KOHOUTKOVÁ, Marcela; Andrea KORIMOVÁ a Petr DUBOVÝ. Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators. In Morphology 2017 50th International Congress of the Czech Anatomical Society 54th Lojda Symposium in Histochemistry, 2017. 2017. ISBN 978-80-263-1315-1.

@proceedings{1389983,
   author = {Kohoutková, Marcela and Korimová, Andrea and Dubový, Petr},
   booktitle = {Morphology 2017 50th International Congress of the Czech Anatomical Society 54th Lojda Symposium in Histochemistry, 2017},
   keywords = {Lipopolysaccharide; Schwann cell; neuroinflammation},
   language = {eng},
   isbn = {978-80-263-1315-1},
   title = {Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators},
   url = {http://www.morphology2017.cz/programme},
   year = {2017}
}

TY  - CONF
ID  - 1389983
AU  - Kohoutková, Marcela - Korimová, Andrea - Dubový, Petr
PY  - 2017
TI  - Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators
SN  - 9788026313151
KW  - Lipopolysaccharide
KW  - Schwann cell
KW  - neuroinflammation
UR  - http://www.morphology2017.cz/programme
L2  - http://www.morphology2017.cz/programme
N2  - Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators Kohoutková M. (1), Korimová A. (1), Dubový P. (1) (1) Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic Wallerian degeneration is a process of stereotypical reactions of Schwann cells which is important prerequisite for reparation of the damage nerve. Inflammatory activation of Schwann cells can be triggered by endogenous ligands produced during Wallerian degeneration or exogenous pathological molecules via Toll-like receptors (TLRs) [1,2]. Lipopolysaccharide (LPS) was used as a prototypical ligand for activation intracellular immune response of schwannoma cells. Acute inflammatory response of schwannoma cells is associated with increased production of cytokines and transcription factors [3]. We studied proteins the most often associated with inflammation – STAT3 and IL-6 and receptor TLR4, which can transduce inflammatory signal to cell. Rat schwannoma cells (RT4-D6P2T) were treated with LPS (10ng/ml) for 1 hour to induce acute inflammatory responses. TLR4, pSTAT3 and IL-6 proteins were studied by immunocytochemistry and Western blot analysis. We observed dynamic changes of TLR4 deposits in the cell cytoplasm but not in the cell surface. LPS treatment induced enlarging of early endosomes in schwannoma cells. We also found significantly increased levels of pSTAT3 and IL-6 proteins after LPS treatment indicating their crucial role in the induction of inflammation cascade in Schwann cells. [1] S. Rotshenker, Wallerian degeneration: the innate-immune response to traumatic nerve injury., J. Neuroinflammation. 8 (2011) 109. [2] E. Ydens, G. Lornet, V. Smits, S. Goethals, V. Timmerman, S. Janssens, The neuroinflammatory role of Schwann cells in disease, Neurobiol. Dis. 55 (2013) 95–103. [3] H.N. Hao, J.D. Peduzzi-Nelson, P.J. VandeVord, K. Barami, S.P. DeSilva, D. Pelinkovic, L.G. Morawa, Lipopolysaccharide-induced inflammatory cytokine production by Schwann’s cells dependent upon TLR4 expression, J. Neuroimmunol. 212 (2009) 26– 34. Supported by grant 16-08508S of The Czech Science Foundation.
ER  -

KOHOUTKOVÁ, Marcela; Andrea KORIMOVÁ a Petr DUBOVÝ. Lipopolysaccharide treatment of schwannoma cells induced acute elevation of inflammatory mediators. In \textit{Morphology 2017 50th International Congress of the Czech Anatomical Society 54th Lojda Symposium in Histochemistry, 2017}. 2017. ISBN~978-80-263-1315-1.

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