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@proceedings{1390077,
author = {Kašný, Martin and Ilgová, Jana and Vorel, Jiří and Roudnický, Pavel and Mikeš, Libor and Dvořáková, Hana and Jedličková, Lucie and Jirsová, Dagmar and Strnad, Hynek and Leontovyč, Roman and Koubková, Božena and Dzika, Ewa and Vetešník, Lukáš and Jurajda, Pavel and Gelnar, Milan},
booktitle = {8th International Symposium on Monogenea},
keywords = {diplozoon; monogenea; molecular interaction; sequencing; transcriptom; secretom;},
language = {eng},
isbn = {978-80-210-8666-1},
title = {Diplozoons (Monogenea): what is the molecular base of their interaction with the host?},
url = {http://ecip.cz/news/4},
year = {2017}
}
TY - CONF
ID - 1390077
AU - Kašný, Martin - Ilgová, Jana - Vorel, Jiří - Roudnický, Pavel - Mikeš, Libor - Dvořáková, Hana - Jedličková, Lucie - Jirsová, Dagmar - Strnad, Hynek - Leontovyč, Roman - Koubková, Božena - Dzika, Ewa - Vetešník, Lukáš - Jurajda, Pavel - Gelnar, Milan
PY - 2017
TI - Diplozoons (Monogenea): what is the molecular base of their interaction with the host?
SN - 9788021086661
KW - diplozoon
KW - monogenea
KW - molecular interaction
KW - sequencing
KW - transcriptom
KW - secretom;
UR - http://ecip.cz/news/4
L2 - http://ecip.cz/news/4
N2 - The representatives from the class Monogenea are predominantly ectoparasites of fish. We adopted the experimental organisms - Eudiplozoon nipponicum (Diplozoidae, Heteronchoinea); a blood-feeding monogenean inhabiting gills of common carp (Cyprinus carpio). In contrast to the other groups of platyhelminths, the identity of genes, the level of their expression and spectrum of functional protein molecules produced by these parasites are very limited. The pyrosequencing methodology (454/Roche, MiSeq/HiSeq) enabled us to generate robust genomic/trancriptomic data and combined them with the results of comprehensive mass spectrometry analysis (Orbitrap-Elite MS System) of excretory-secretory products isolated from the adult worms. The pipelines of bioinformatic data processing were optimized and number of functional molecules was annotated. The reached platform currently represents significant source of information available for further experimental research; up to now at least six functional protein molecules (peptidases and their inhibitors) have been molecularly / biochemically / immunochemically characterized.
ER -
KAŠNÝ, Martin, Jana ILGOVÁ, Jiří VOREL, Pavel ROUDNICKÝ, Libor MIKEŠ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ, Dagmar JIRSOVÁ, Hynek STRNAD, Roman LEONTOVYČ, Božena KOUBKOVÁ, Ewa DZIKA, Lukáš VETEŠNÍK, Pavel JURAJDA a Milan GELNAR. Diplozoons (Monogenea): what is the molecular base of their interaction with the host? In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.
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