Application of new organometallic compounds on ovarian cancer
cell lines.

k 2017

Application of new organometallic compounds on ovarian cancer cell lines.

SKOUPILOVÁ, Hana; Jindrich KARBAN; Jiri PINKAS; Martin BARTOŠÍK; Michaela VAŠINOVÁ GALIOVÁ et al.

Základní údaje

Originální název

Application of new organometallic compounds on ovarian cancer cell lines.

Název česky

Aplikace nových organokovových sloučenin na ovariálních buněčných liniích

Autoři

SKOUPILOVÁ, Hana; Jindrich KARBAN; Jiri PINKAS; Martin BARTOŠÍK; Michaela VAŠINOVÁ GALIOVÁ a Roman HRSTKA

Vydání

Chemistry and biology of phytohormones and related substances. 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00097633

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

ferrocenes; metallocenes; organometallic compounds; cancer therapy

Příznaky

Mezinárodní význam

Změněno: 22. 8. 2019 13:00, Mgr. Hana Kubušová, Ph.D.

Anotace

V originále

The synthesis of compounds with the central metal atom in its structure represents very up-todate and attractive trend in anticancer drug development programmes. The first such compound is a cisplatin, whose cytotoxic effects were discovered in 1967 by Barnett Rosenberg (1). In spite of the indisputable success against some types of human malignancies, cisplatin as well as its derivatives displays a number of side effects such as nephrotoxicity, haemolytic anemia and other. Important problems are also toxicity towards normal fast dividing cells and progression of drug resistance during the therapy (2). The limitations of cisplatin started research focused on other transition metal complexes different from platinum, e.g. Fe, Ru, Ti, Zr, etc., which as we believe may in near future substitute cisplatin-based antitumor therapy. In collaboration with several laboratories a series of new organometallic complexes containing iron in their structure was synthesised. The cytotoxicity of these compounds was then monitored on selected ovarian cancer cell lines A2780, A2780cis and SK-OV-3 (3, 4, unpublished data). Compounds with the highest cytotoxic effect have been chosen to study their ability to cumulate inside the cells. We developed a new method based on differential pulse voltammetry (DPV), to determine the amount of accumulated drugs. These data were compared with the results obtained from ICP-MS, which is ordinarily used to detect metals at very low concentrations. Both methods confirmed elevated levels of iron in tested cancer cells (4, unpublished data). Based on this, DPV was used to determine the processes responsible for cellular uptake of selected compounds more in detail. Another important goal is to find out the mechanism by which ferrocenes operate in a cancer cells. Our preliminary experiments indicate an increased development of oxygen radicals that may represent a key cytotoxic mechanism of action induced by these drugs.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SKOUPILOVÁ, Hana; Jindrich KARBAN; Jiri PINKAS; Martin BARTOŠÍK; Michaela VAŠINOVÁ GALIOVÁ a Roman HRSTKA. Application of new organometallic compounds on ovarian cancer cell lines. In Chemistry and biology of phytohormones and related substances. 2017.

@proceedings{1390147,
   author = {Skoupilová, Hana and Karban, Jindrich and Pinkas, Jiri and Bartošík, Martin and Vašinová Galiová, Michaela and Hrstka, Roman},
   booktitle = {Chemistry and biology of phytohormones and related substances.},
   keywords = {ferrocenes; metallocenes; organometallic compounds; cancer therapy},
   language = {eng},
   title = {Application of new organometallic compounds on ovarian cancer cell lines.},
   year = {2017}
}

TY  - CONF
ID  - 1390147
AU  - Skoupilová, Hana - Karban, Jindrich - Pinkas, Jiri - Bartošík, Martin - Vašinová Galiová, Michaela - Hrstka, Roman
PY  - 2017
TI  - Application of new organometallic compounds on ovarian cancer cell lines.
KW  - ferrocenes
KW  - metallocenes
KW  - organometallic compounds
KW  - cancer therapy
N2  - The synthesis of compounds with the central metal atom in its structure represents very up-todate and attractive trend in anticancer drug development programmes. The first such compound is a cisplatin, whose cytotoxic effects were discovered in 1967 by Barnett Rosenberg (1). In spite of the indisputable success against some types of human malignancies, cisplatin as well as its derivatives displays a number of side effects such as nephrotoxicity, haemolytic anemia and other. Important problems are also toxicity towards normal fast dividing cells and progression of drug resistance during the therapy (2). The limitations of cisplatin started research focused on other transition metal complexes different from platinum, e.g. Fe, Ru, Ti, Zr, etc., which as we believe may in near future substitute cisplatin-based antitumor therapy. In collaboration with several laboratories a series of new organometallic complexes containing iron in their structure was synthesised. The cytotoxicity of these compounds was then monitored on selected ovarian cancer cell lines A2780, A2780cis and SK-OV-3 (3, 4, unpublished data). Compounds with the highest cytotoxic effect have been chosen to study their ability to cumulate inside the cells. We developed a new method based on differential pulse voltammetry (DPV), to determine the amount of accumulated drugs. These data were compared with the results obtained from ICP-MS, which is ordinarily used to detect metals at very low concentrations. Both methods confirmed elevated levels of iron in tested cancer cells (4, unpublished data). Based on this, DPV was used to determine the processes responsible for cellular uptake of selected compounds more in detail. Another important goal is to find out the mechanism by which ferrocenes operate in a cancer cells. Our preliminary experiments indicate an increased development of oxygen radicals that may represent a key cytotoxic mechanism of action induced by these drugs.
ER  -

SKOUPILOVÁ, Hana; Jindrich KARBAN; Jiri PINKAS; Martin BARTOŠÍK; Michaela VAŠINOVÁ GALIOVÁ a Roman HRSTKA. Application of new organometallic compounds on ovarian cancer cell lines. In \textit{Chemistry and biology of phytohormones and related substances.}. 2017.

Přehled o publikaci