GENOTYPING OF EIGHT  TRICHINELLA  SPECIES USING HIGH 
RESOLUTION  MELTING ANALYSIS

a 2017

GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS

ŠKORPÍKOVÁ, Lucie; Nikol RESLOVÁ; Michal SLANÝ; Edoardo POZIO; Martin KAŠNÝ et al.

Základní údaje

Originální název

GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS

Název česky

Genotypizace osmi druhů trichinel pomocí analýzy tání s vysokým rozlišením (high resolution melting analysis).

Autoři

ŠKORPÍKOVÁ, Lucie; Nikol RESLOVÁ; Michal SLANÝ; Edoardo POZIO a Martin KAŠNÝ

Vydání

23rd Helminthological Days, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00097650

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-968473-8-9

Klíčová slova česky

Trichinella; genotypizace; high resolution melting analysis; HRMA

Klíčová slova anglicky

Trichinella; genotyping; high resolution melting analysis; HRMA

Příznaky

Mezinárodní význam

Změněno: 15. 3. 2018 12:18, Mgr. Nikol Reslová, Ph.D.

Anotace

V originále

Nematodes of the genus Trichinella are worldwide distributed foodborne parasites with the high impact on public health and animal trade. They invade small intestine cells and skeletal muscles of wide range of mammals, birds and reptiles. Trichinella spp. are causative agents of human trichinellosis, a serious disease which has been documented in 55 countries with an average incidence of approximately ten-thousand cases per year. According to ability of muscle larvae to form a collagen capsule, within the genus Trichinella two main clades are recognized. The “encapsulated clade” contains six species (T1 - Trichinella spiralis, T2 - T. nativa, T3 - T. britovi, T5 - T. murrelli, T7 - T. nelsoni, T12 - T. patagoniensis) and three additional taxonomically undefined genotypes (T6, T8 and T9). “Non-encapsulated clade” includes three species (T4 - T. pseudospiralis, T10 - T. papuae and T11 - T. zimbabwensis). Despite the difference in capsule formation and some size differences, the species and genotypes of all developmental stages of these parasites are morphologically indistinguishably. Therefore, we have developed a high resolution melting analysis (HRMA) for the unequivocal differentiation of eight Trichinella species (T1, T2, T3, T4, T5, T7, T10 and T11). The assay was based on the mitochondrial cytochrome c oxidase subunit I (COI) gene, representing the variable part of DNA sequence which shows high sequence divergence even among closely allied species. The genomic DNA of a single muscle larva was used as a template in qPCR, the polymorphic region of the COI gene was amplified and the obtained amplicons were analysed for species-specific HRMA curves.

Návaznosti

MUNI/A/1362/2016, interní kód MU

Název: Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí

Investor: Masarykova univerzita, Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ŠKORPÍKOVÁ, Lucie; Nikol RESLOVÁ; Michal SLANÝ; Edoardo POZIO a Martin KAŠNÝ. GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS. In 23rd Helminthological Days. 2017. ISBN 978-80-968473-8-9.

@proceedings{1390257,
   author = {Škorpíková, Lucie and Reslová, Nikol and Slaný, Michal and Pozio, Edoardo and Kašný, Martin},
   booktitle = {23rd Helminthological Days},
   keywords = {Trichinella; genotyping; high resolution melting analysis; HRMA},
   language = {eng},
   isbn = {978-80-968473-8-9},
   title = {GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS},
   url = {http://pau.saske.sk/helminthdays2017/},
   year = {2017}
}

TY  - CONF
ID  - 1390257
AU  - Škorpíková, Lucie - Reslová, Nikol - Slaný, Michal - Pozio, Edoardo - Kašný, Martin
PY  - 2017
TI  - GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS
SN  - 9788096847389
KW  - Trichinella
KW  - genotyping
KW  - high resolution melting analysis
KW  - HRMA
UR  - http://pau.saske.sk/helminthdays2017/
N2  - Nematodes of the genus Trichinella are worldwide distributed foodborne parasites with the high impact on public health and animal trade. They invade small intestine cells and skeletal muscles of wide range of mammals, birds and reptiles. Trichinella spp. are causative agents of human trichinellosis, a serious disease which has been documented in 55 countries with an average incidence of approximately ten-thousand cases per year. According to ability of muscle larvae to form a collagen capsule, within the genus Trichinella two main clades are recognized. The “encapsulated clade” contains six species (T1 - Trichinella spiralis, T2 - T. nativa, T3 - T. britovi, T5 - T. murrelli, T7 - T. nelsoni, T12 - T. patagoniensis) and three additional taxonomically undefined genotypes (T6, T8 and T9). “Non-encapsulated clade” includes three species (T4 - T. pseudospiralis, T10 - T. papuae and T11 - T. zimbabwensis). Despite the difference in capsule formation and some size differences, the species and genotypes of all developmental stages of these parasites are morphologically indistinguishably. Therefore, we have developed a high resolution melting analysis (HRMA) for the unequivocal differentiation of eight Trichinella species (T1, T2, T3, T4, T5, T7, T10 and T11). The assay was based on the mitochondrial cytochrome c oxidase subunit I (COI) gene, representing the variable part of DNA sequence which shows high sequence divergence even among closely allied species. The genomic DNA of a single muscle larva was used as a template in qPCR, the polymorphic region of the COI gene was amplified and the obtained amplicons were analysed for species-specific HRMA curves.
ER  -

ŠKORPÍKOVÁ, Lucie; Nikol RESLOVÁ; Michal SLANÝ; Edoardo POZIO a Martin KAŠNÝ. GENOTYPING OF EIGHT TRICHINELLA SPECIES USING HIGH RESOLUTION MELTING ANALYSIS. In \textit{23rd Helminthological Days}. 2017. ISBN~978-80-968473-8-9.

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