Francisella tularensis prevalence and load in Dermacentor
reticulatus ticks in an endemic area in Central Europe

J 2017

Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe

HUBÁLEK, Zdeněk a Ivo RUDOLF

Základní údaje

Originální název

Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe

Název česky

Prevalence a "nálož" Francisella tularensis v klíšťatech Dermacentor reticulatus v endemické oblasti střední Evropy

Autoři

HUBÁLEK, Zdeněk a Ivo RUDOLF

Vydání

MEDICAL AND VETERINARY ENTOMOLOGY, HOBOKEN, WILEY, 2017, 0269-283X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.688

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1111/mve.12229

UT WoS

000400031700015

Klíčová slova česky

klíšťata+ nálož patogena; tularemie

Klíčová slova anglicky

Ixodid ticks; pathogen load; tularaemia

Štítky

RIV ne

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2020 14:56, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

A total of 7778 host-seeking adult Dermacentor reticulatus (Ixodida: Ixodidae) ticks were examined for the prevalence of Francisella tularensis holarctica (Thiotrichales: Francisellaceae) in a natural focus of tularaemia in the floodplain forest-meadow ecosystem along the lower reaches of the Dyje (Thaya) river in South Moravia (Czech Republic) between 1995 and 2013. Ticks were pooled (10 specimens per pool) and their homogenates inoculated subcutaneously in 4-week-old specific pathogen-free mice. Dead mice were sectioned, their spleens cultivated on thioglycollate-glucose-blood agar and impression smears from the spleen, liver and heart blood were Giemsa-stained. Sixty-four pools were positive for F.tularensis: the overall minimum infection rate (MIR) was 0.82%. Overall MIRs for the 4714 female and 3064 male D.reticulatus examined were 0.89 and 0.72%, respectively; MIRs fluctuated across years between 0.0 and 2.43%. The estimated bacterial load in infected ticks varied from 0.84 to 5.34 log(10) infectious F.tularensis cells per tick (i.e. from about seven to 220000 cells). Ticks with low loads were more prevalent; more than 1000 infectious cells were detected in 24 ticks (0.3% of all ticks and 37.5% of infected ticks). Monitoring of D.reticulatus for the presence and cell numbers of F.tularensis may be a valuable tool in the surveillance of tularaemia.

Citovat

HUBÁLEK, Zdeněk a Ivo RUDOLF. Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe. MEDICAL AND VETERINARY ENTOMOLOGY. HOBOKEN: WILEY, 2017, roč. 31, č. 2, s. 234-239. ISSN 0269-283X. Dostupné z: https://doi.org/10.1111/mve.12229.

@article{1390265,
   author = {Hubálek, Zdeněk and Rudolf, Ivo},
   article_location = {HOBOKEN},
   article_number = {2},
   doi = {https://doi.org/10.1111/mve.12229},
   keywords = {Ixodid ticks; pathogen load; tularaemia},
   language = {eng},
   issn = {0269-283X},
   journal = {MEDICAL AND VETERINARY ENTOMOLOGY},
   note = {Žádný z autorů nemá afiliaci na MU.},
   title = {Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe},
   url = {http://dx.doi.org/10.1111/mve.12229},
   volume = {31},
   year = {2017}
}

TY  - JOUR
ID  - 1390265
AU  - Hubálek, Zdeněk - Rudolf, Ivo
PY  - 2017
TI  - Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe
JF  - MEDICAL AND VETERINARY ENTOMOLOGY
VL  - 31
IS  - 2
SP  - 234-239
EP  - 234-239
PB  - WILEY
SN  - 0269283X
N1  - Žádný z autorů nemá afiliaci na MU.
KW  - Ixodid ticks
KW  - pathogen load
KW  - tularaemia
UR  - http://dx.doi.org/10.1111/mve.12229
L2  - http://dx.doi.org/10.1111/mve.12229
N2  - A total of 7778 host-seeking adult Dermacentor reticulatus (Ixodida: Ixodidae) ticks were examined for the prevalence of Francisella tularensis holarctica (Thiotrichales: Francisellaceae) in a natural focus of tularaemia in the floodplain forest-meadow ecosystem along the lower reaches of the Dyje (Thaya) river in South Moravia (Czech Republic) between 1995 and 2013. Ticks were pooled (10 specimens per pool) and their homogenates inoculated subcutaneously in 4-week-old specific pathogen-free mice. Dead mice were sectioned, their spleens cultivated on thioglycollate-glucose-blood agar and impression smears from the spleen, liver and heart blood were Giemsa-stained. Sixty-four pools were positive for F.tularensis: the overall minimum infection rate (MIR) was 0.82%. Overall MIRs for the 4714 female and 3064 male D.reticulatus examined were 0.89 and 0.72%, respectively; MIRs fluctuated across years between 0.0 and 2.43%. The estimated bacterial load in infected ticks varied from 0.84 to 5.34 log(10) infectious F.tularensis cells per tick (i.e. from about seven to 220000 cells). Ticks with low loads were more prevalent; more than 1000 infectious cells were detected in 24 ticks (0.3% of all ticks and 37.5% of infected ticks). Monitoring of D.reticulatus for the presence and cell numbers of F.tularensis may be a valuable tool in the surveillance of tularaemia.
ER  -

HUBÁLEK, Zdeněk a Ivo RUDOLF. Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe. \textit{MEDICAL AND VETERINARY ENTOMOLOGY}. HOBOKEN: WILEY, 2017, roč.~31, č.~2, s.~234-239. ISSN~0269-283X. Dostupné z: https://doi.org/10.1111/mve.12229.

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