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@proceedings{1390852,
author = {Levin, Shahaf and Kubíčková, Kateřina and Klusáková, Ilona and Dubový, Petr and Joukal, Marek},
booktitle = {Morphology 2017},
language = {eng},
isbn = {978-80-263-1315-1},
title = {TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment},
year = {2017}
}
TY - CONF
ID - 1390852
AU - Levin, Shahaf - Kubíčková, Kateřina - Klusáková, Ilona - Dubový, Petr - Joukal, Marek
PY - 2017
TI - TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment
SN - 9788026313151
N2 - Chemotherapeutic agent Paclitaxel causes peripheral neuropathy based on alteration of calcium channels and defragmentation of mitochondria in peripheral nerve axons. These changes are related to the release of damage associated molecular patterns (DAMPs) which are associated with the expression of Toll-like receptor 9 (TLR-9). The aim of our study was to assess the changes of TLR-9 in the dorsal root ganglia (DRGs), related to Paclitaxel application. Wistar rats (n=18, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received vehiculum (alcohol and cremophor; 1:1). The animals were left to survive for 1, 3 and 7 days from the last application. Experimental and control rats were sacrificed together with naïve rats and perfused transcardially by Zamboni´s fixative. Longitudinal cryostat sections through the lumbar and cervical DRGs were cut and immunostained for TLR-9. Satellite glial cells (SGCs) were visualized using GFAP antibody. The intensity of TLR-9 immunofluorescence in the DRG was measured and statistically analyzed. The presence of TLR-9 immunofluorescence was found in the cytoplasm of both small-sized neurons and of satellite glial cells surrounding large-sized neurons, in both naïve and control animals. However, the application of Paclitaxel caused a visible presence of TLR-9 in medium-sized neurons as well. Statistically significant increase in TLR-9 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to control and naïve animals. The intensity of TLR-9 immunofluorescence increased with time of survival after the last application. Our results clearly indicate that DRGs react to Paclitaxel application with an increase of TLR-9 expression. These changes might be associated with inflammatory reaction in chemotherapy induced peripheral neuropathy.
ER -
LEVIN, Shahaf, Kateřina KUBÍČKOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment. In \textit{Morphology 2017}. 2017. ISBN~978-80-263-1315-1.
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