Detection of let-7 miRNAs in urine supernatant as potential
diagnostic approach in non-metastatic clear-cell renal cell
carcinoma

J 2017

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

FEDORKO, Michal; Jaroslav JURÁČEK; Michal STANÍK; Marek SVOBODA; Alexandr POPRACH et. al.

Základní údaje

Originální název

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Název česky

Detekce let-7 miRNA v močovém supernatantu jako potenciální způsob diagnostiky nemetastatického karcinomu z renálních buněk

Autoři

FEDORKO, Michal; Jaroslav JURÁČEK; Michal STANÍK; Marek SVOBODA; Alexandr POPRACH; Tomáš BÜCHLER; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ

Vydání

Biochemia Medica, Zagreb, CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, 1330-0962

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Chorvatsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.653

Kód RIV

RIV/00216224:14110/17:00095662

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.11613/BM.2017.043

UT WoS

000404505700018

EID Scopus

2-s2.0-85020673994

Klíčová slova česky

karcinom z renálních buněk; močové miRNA; let-7; diagnostický biomarker

Klíčová slova anglicky

renal cell carcinoma; urinary miRNAs; let-7; diagnostic biomarker

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 17:37, Soňa Böhmová

Anotace

ORIG CZ

V originále

Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.

Česky

MikroRNA v moči jsou potenciálním nástrojem pro časnou a neinvazivní detekci různých typů maligních nádorů. Cílem studie bylo zhodnotit, zda se liší koncentrace miRNA z rodiny let-7 v moči pacientů s renálním karcinomem od zdravých kontrol.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-31071A, projekt VaV

Název: Studium močových/tkáňových mikroRNA jako potenciálních biomarkerů uroteliálního karcinomu močového měchýře

NV15-34678A, projekt VaV

Název: Molekulární prognostické a prediktivní faktory u pacientů s metastatickým renálním karcinomem léčených tyrozinkinázovými inhibitory

Citovat

FEDORKO, Michal; Jaroslav JURÁČEK; Michal STANÍK; Marek SVOBODA; Alexandr POPRACH; Tomáš BÜCHLER; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ. Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma. Biochemia Medica. Zagreb: CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, roč. 27, č. 2, s. 411-417. ISSN 1330-0962. Dostupné z: https://doi.org/10.11613/BM.2017.043.

@article{1391715,
   author = {Fedorko, Michal and Juráček, Jaroslav and Staník, Michal and Svoboda, Marek and Poprach, Alexandr and Büchler, Tomáš and Pacík, Dalibor and Doležel, Jan and Slabý, Ondřej},
   article_location = {Zagreb},
   article_number = {2},
   doi = {https://doi.org/10.11613/BM.2017.043},
   keywords = {renal cell carcinoma; urinary miRNAs; let-7; diagnostic biomarker},
   language = {eng},
   issn = {1330-0962},
   journal = {Biochemia Medica},
   title = {Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma},
   volume = {27},
   year = {2017}
}

TY  - JOUR
ID  - 1391715
AU  - Fedorko, Michal - Juráček, Jaroslav - Staník, Michal - Svoboda, Marek - Poprach, Alexandr - Büchler, Tomáš - Pacík, Dalibor - Doležel, Jan - Slabý, Ondřej
PY  - 2017
TI  - Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
JF  - Biochemia Medica
VL  - 27
IS  - 2
SP  - 411-417
EP  - 411-417
PB  - CROATIAN SOC MEDICAL BIOCHEMISTS
SN  - 13300962
KW  - renal cell carcinoma
KW  - urinary miRNAs
KW  - let-7
KW  - diagnostic biomarker
N2  - Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
ER  -

FEDORKO, Michal; Jaroslav JURÁČEK; Michal STANÍK; Marek SVOBODA; Alexandr POPRACH; Tomáš BÜCHLER; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ. Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma. \textit{Biochemia Medica}. Zagreb: CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, roč.~27, č.~2, s.~411-417. ISSN~1330-0962. Dostupné z: https://doi.org/10.11613/BM.2017.043.

Přehled o publikaci