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@article{1391715,
author = {Fedorko, Michal and Juráček, Jaroslav and Staník, Michal and Svoboda, Marek and Poprach, Alexandr and Büchler, Tomáš and Pacík, Dalibor and Doležel, Jan and Slabý, Ondřej},
article_location = {Zagreb},
article_number = {2},
doi = {http://dx.doi.org/10.11613/BM.2017.043},
keywords = {renal cell carcinoma; urinary miRNAs; let-7; diagnostic biomarker},
language = {eng},
issn = {1330-0962},
journal = {Biochemia Medica},
title = {Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma},
volume = {27},
year = {2017}
}
TY - JOUR
ID - 1391715
AU - Fedorko, Michal - Juráček, Jaroslav - Staník, Michal - Svoboda, Marek - Poprach, Alexandr - Büchler, Tomáš - Pacík, Dalibor - Doležel, Jan - Slabý, Ondřej
PY - 2017
TI - Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
JF - Biochemia Medica
VL - 27
IS - 2
SP - 411-417
EP - 411-417
PB - CROATIAN SOC MEDICAL BIOCHEMISTS
SN - 13300962
KW - renal cell carcinoma
KW - urinary miRNAs
KW - let-7
KW - diagnostic biomarker
N2 - Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
ER -
FEDORKO, Michal, Jaroslav JURÁČEK, Michal STANÍK, Marek SVOBODA, Alexandr POPRACH, Tomáš BÜCHLER, Dalibor PACÍK, Jan DOLEŽEL a Ondřej SLABÝ. Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma. \textit{Biochemia Medica}. Zagreb: CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, roč.~27, č.~2, s.~411-417. ISSN~1330-0962. Dostupné z: https://dx.doi.org/10.11613/BM.2017.043.
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