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@article{1392284,
author = {Schejbal, Jan and Glatz, Zdeněk},
article_location = {Německo},
article_number = {1},
doi = {http://dx.doi.org/10.1002/jssc.201700905},
keywords = {capillary electrophoresis; drug metabolism; druggable enzymes; immobilized- enzyme reactors},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research},
url = {http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700905/abstract?campaign=wolacceptedarticle},
volume = {41},
year = {2018}
}
TY - JOUR
ID - 1392284
AU - Schejbal, Jan - Glatz, Zdeněk
PY - 2018
TI - Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research
JF - Journal of Separation Science
VL - 41
IS - 1
SP - 323-335
EP - 323-335
PB - Weinheim : WILEY-VCH Verlag GmbH & Co
SN - 16159306
KW - capillary electrophoresis
KW - drug metabolism
KW - druggable enzymes
KW - immobilized- enzyme reactors
UR - http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700905/abstract?campaign=wolacceptedarticle
L2 - http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700905/abstract?campaign=wolacceptedarticle
N2 - Enzymes play an essential role in many aspects of pharmaceutical research as drug targets, drug metabolizers, enzyme drugs and more. In this specific field, enzyme assays are required to meet a number of specific requirements, such as low cost, easy automation and high reliability. The integration of an immobilized-enzyme reactor to capillary electrophoresis represents a unique approach to fulfilling these criteria by combining the benefits of enzyme immobilization, that is, increased stability and repeated use, as well as the minute sample consumption, short analysis time and efficient analysis provided by capillary electrophoresis. In this review, we summarize, analyse and discuss published works where pharmaceutically relevant enzymes were used to prepare capillary-electrophoresis-integrated immobilized-enzyme reactors in an online manner. The presented assays are divided into three distinct groups based on the drug–enzyme relationship. The first, more extensively studied group employs enzymes which are considered to be therapeutic targets, the second group of assays presents tools to assess drug metabolism and the third group assesses enzyme drugs. Furthermore, we examine various methods of enzyme immobilization and their implications for assay properties.
ER -
SCHEJBAL, Jan a Zdeněk GLATZ. Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research. \textit{Journal of Separation Science}. Německo: Weinheim : WILEY-VCH Verlag GmbH \&{} Co, 2018, roč.~41, č.~1, s.~323-335. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201700905.
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