Spectroscopic insights into quadruplexes of five-repeat
telomere DNA sequences upon G-block damage.

J 2017

Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage.

DVOŘÁKOVÁ, Zuzana; Michaela VORLÍČKOVÁ a Daniel RENČIUK

Základní údaje

Originální název

Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage.

Autoři

DVOŘÁKOVÁ, Zuzana; Michaela VORLÍČKOVÁ a Daniel RENČIUK

Vydání

Biochim. Biophys. Acta, 2017, 0005-2736

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.438

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Abazické poškození, Cirkulární dichroizmus, Poškození DNA, Guaninové kvadruplexy, Lidská telomera

Klíčová slova anglicky

Abasic lesion; Circular dichroism; DNA mutation; Guanine quadruplex; Human telomere

Štítky

RIV ne

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2020 14:50, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

BACKGROUND: The DNA lesions, resulting from oxidative damage, were shown to destabilize human telomere four-repeat quadruplex and to alter its structure. Long telomere DNA, as a repetitive sequence, offers, however, other mechanisms of dealing with the lesion: extrusion of the damaged repeat into loop or shifting the quadruplex position by one repeat. METHODS: Using circular dichroism and UV absorption spectroscopy and polyacrylamide electrophoresis, we studied consequences of lesions at different positions of the model five-repeat human telomere DNA sequences on the structure and stability of their quadruplexes in sodium and in potassium. RESULTS: The repeats affected by lesion are preferentially positioned as terminal overhangs of the core quadruplex structurally similar to the four-repeat one. Forced affecting of the inner repeats leads to presence of variety of more parallel folds in potassium. In sodium the designed models form mixture of two dominant antiparallel quadruplexes whose population varies with the position of the affected repeat. The shapes of quadruplex CD spectra, namely the height of dominant peaks, significantly correlate with melting temperatures. CONCLUSION: Lesion in one guanine tract of a more than four repeats long human telomere DNA sequence may cause re-positioning of its quadruplex arrangement associated with a shift of the structure to less common quadruplex conformations. The type of the quadruplex depends on the loop position and external conditions. GENERAL SIGNIFICANCE: The telomere DNA quadruplexes are quite resistant to the effect of point mutations due to the telomere DNA repetitive nature, although their structure and, consequently, function might be altered.

Citovat

DVOŘÁKOVÁ, Zuzana; Michaela VORLÍČKOVÁ a Daniel RENČIUK. Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage. Biochim. Biophys. Acta. 2017, roč. 1861, č. 11, s. 2750-2757. ISSN 0005-2736. Dostupné z: https://doi.org/10.1016/j.bbagen.2017.07.019.

@article{1392892,
   author = {Dvořáková, Zuzana and Vorlíčková, Michaela and Renčiuk, Daniel},
   article_number = {11},
   doi = {https://doi.org/10.1016/j.bbagen.2017.07.019},
   keywords = {Abasic lesion; Circular dichroism; DNA mutation; Guanine quadruplex; Human telomere},
   issn = {0005-2736},
   journal = {Biochim. Biophys. Acta},
   note = {Žádný z autorů nemá afiliaci na MU.},
   title = {Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage.},
   url = {http://www.sciencedirect.com/science/article/pii/S0304416517302362},
   volume = {1861},
   year = {2017}
}

TY  - JOUR
ID  - 1392892
AU  - Dvořáková, Zuzana - Vorlíčková, Michaela - Renčiuk, Daniel
PY  - 2017
TI  - Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage.
JF  - Biochim. Biophys. Acta
VL  - 1861
IS  - 11
SP  - 2750-2757
EP  - 2750-2757
SN  - 00052736
N1  - Žádný z autorů nemá afiliaci na MU.
KW  - Abasic lesion
KW  - Circular dichroism
KW  - DNA mutation
KW  - Guanine quadruplex
KW  - Human telomere
UR  - http://www.sciencedirect.com/science/article/pii/S0304416517302362
N2  - BACKGROUND: The DNA lesions, resulting from oxidative damage, were shown to destabilize human telomere four-repeat quadruplex and to alter its structure. Long telomere DNA, as a repetitive sequence, offers, however, other mechanisms of dealing with the lesion: extrusion of the damaged repeat into loop or shifting the quadruplex position by one repeat. METHODS: Using circular dichroism and UV absorption spectroscopy and polyacrylamide electrophoresis, we studied consequences of lesions at different positions of the model five-repeat human telomere DNA sequences on the structure and stability of their quadruplexes in sodium and in potassium. RESULTS: The repeats affected by lesion are preferentially positioned as terminal overhangs of the core quadruplex structurally similar to the four-repeat one. Forced affecting of the inner repeats leads to presence of variety of more parallel folds in potassium. In sodium the designed models form mixture of two dominant antiparallel quadruplexes whose population varies with the position of the affected repeat. The shapes of quadruplex CD spectra, namely the height of dominant peaks, significantly correlate with melting temperatures. CONCLUSION: Lesion in one guanine tract of a more than four repeats long human telomere DNA sequence may cause re-positioning of its quadruplex arrangement associated with a shift of the structure to less common quadruplex conformations. The type of the quadruplex depends on the loop position and external conditions. GENERAL SIGNIFICANCE: The telomere DNA quadruplexes are quite resistant to the effect of point mutations due to the telomere DNA repetitive nature, although their structure and, consequently, function might be altered.
ER  -

DVOŘÁKOVÁ, Zuzana; Michaela VORLÍČKOVÁ a Daniel RENČIUK. Spectroscopic insights into quadruplexes of five-repeat telomere DNA sequences upon G-block damage. \textit{Biochim. Biophys. Acta}. 2017, roč.~1861, č.~11, s.~2750-2757. ISSN~0005-2736. Dostupné z: https://doi.org/10.1016/j.bbagen.2017.07.019.

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