Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana

J 2017

Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana

RABANAL, Fernando A., Viktoria NIZHYNSKA, Terezie MANDÁKOVÁ, Polina Yu NOVIKOVA, Martin LYSÁK et. al.

Základní údaje

Originální název

Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana

Autoři

RABANAL, Fernando A. (40 Rakousko), Viktoria NIZHYNSKA (40 Rakousko), Terezie MANDÁKOVÁ (203 Česká republika, domácí), Polina Yu NOVIKOVA (40 Rakousko), Martin LYSÁK (203 Česká republika, garant, domácí), Richard MOTT (826 Velká Británie a Severní Irsko) a Magnus NORDBORG (40 Rakousko)

Vydání

G3-Genes, Genomes, Genetics, USA, Bethesda, 2017, 2160-1836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.742

Kód RIV

RIV/00216224:14740/17:00095157

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1534/g3.117.040204

UT WoS

000398840700013

Klíčová slova anglicky

ribosomes; 45S rRNA genes; natural variation; Arabidopsis thaliana

Štítky

OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 3. 2018 14:39, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The considerable genome size variation in Arabidopsis thaliana has been shown largely to be due to copy number variation (CNV) in 45S ribosomal RNA (rRNA) genes. Surprisingly, attempts to map this variation by means of genome-wide association studies (GWAS) failed to identify either of the two likely sources, namely the nucleolus organizer regions (NORs). Instead, GWAS implicated a trans-acting locus, as if rRNA gene CNV was a phenotype rather than a genotype. To explain these results, we investigated the inheritance and stability of rRNA gene copy number using the variety of genetic resources available in A. thaliana F2 crosses, recombinant inbred lines, the multiparent advanced-generation inter-cross population, and mutation accumulation lines. Our results clearly show that rRNA gene CNV can be mapped to the NORs themselves, with both loci contributing equally to the variation. However, NOR size is unstably inherited, and dramatic copy number changes are visible already within tens of generations, which explains why it is not possible to map the NORs using GWAS. We did not find any evidence of trans-acting loci in crosses, which is also expected since changes due to such loci would take very many generations to manifest themselves. rRNA gene copy number is thus an interesting example of missing heritabilitya trait that is heritable in pedigrees, but not in the general population.

Návaznosti

GBP501/12/G090, projekt VaV

Název: Evoluce a funkce komplexních genomů rostlin

Citovat

RABANAL, Fernando A., Viktoria NIZHYNSKA, Terezie MANDÁKOVÁ, Polina Yu NOVIKOVA, Martin LYSÁK, Richard MOTT a Magnus NORDBORG. Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana. G3-Genes, Genomes, Genetics. USA: Bethesda, 2017, roč. 7, č. 4, s. 1201-1209. ISSN 2160-1836. Dostupné z: https://dx.doi.org/10.1534/g3.117.040204.

@article{1395015,
   author = {Rabanal, Fernando A. and Nizhynska, Viktoria and Mandáková, Terezie and Novikova, Polina Yu and Lysák, Martin and Mott, Richard and Nordborg, Magnus},
   article_location = {USA},
   article_number = {4},
   doi = {http://dx.doi.org/10.1534/g3.117.040204},
   keywords = {ribosomes; 45S rRNA genes; natural variation; Arabidopsis thaliana},
   language = {eng},
   issn = {2160-1836},
   journal = {G3-Genes, Genomes, Genetics},
   title = {Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana},
   url = {http://www.g3journal.org/content/7/4/1201},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1395015
AU  - Rabanal, Fernando A. - Nizhynska, Viktoria - Mandáková, Terezie - Novikova, Polina Yu - Lysák, Martin - Mott, Richard - Nordborg, Magnus
PY  - 2017
TI  - Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana
JF  - G3-Genes, Genomes, Genetics
VL  - 7
IS  - 4
SP  - 1201-1209
EP  - 1201-1209
PB  - Bethesda
SN  - 21601836
KW  - ribosomes
KW  - 45S rRNA genes
KW  - natural variation
KW  - Arabidopsis thaliana
UR  - http://www.g3journal.org/content/7/4/1201
L2  - http://www.g3journal.org/content/7/4/1201
N2  - The considerable genome size variation in Arabidopsis thaliana has been shown largely to be due to copy number variation (CNV) in 45S ribosomal RNA (rRNA) genes. Surprisingly, attempts to map this variation by means of genome-wide association studies (GWAS) failed to identify either of the two likely sources, namely the nucleolus organizer regions (NORs). Instead, GWAS implicated a trans-acting locus, as if rRNA gene CNV was a phenotype rather than a genotype. To explain these results, we investigated the inheritance and stability of rRNA gene copy number using the variety of genetic resources available in A. thaliana F2 crosses, recombinant inbred lines, the multiparent advanced-generation inter-cross population, and mutation accumulation lines. Our results clearly show that rRNA gene CNV can be mapped to the NORs themselves, with both loci contributing equally to the variation. However, NOR size is unstably inherited, and dramatic copy number changes are visible already within tens of generations, which explains why it is not possible to map the NORs using GWAS. We did not find any evidence of trans-acting loci in crosses, which is also expected since changes due to such loci would take very many generations to manifest themselves. rRNA gene copy number is thus an interesting example of missing heritabilitya trait that is heritable in pedigrees, but not in the general population.
ER  -

RABANAL, Fernando A., Viktoria NIZHYNSKA, Terezie MANDÁKOVÁ, Polina Yu NOVIKOVA, Martin LYSÁK, Richard MOTT a Magnus NORDBORG. Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana. \textit{G3-Genes, Genomes, Genetics}. USA: Bethesda, 2017, roč.~7, č.~4, s.~1201-1209. ISSN~2160-1836. Dostupné z: https://dx.doi.org/10.1534/g3.117.040204.

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