Preparative isoelectric focusing of microorganisms in
cellulose-based separation medium and subsequent analysis by
CIEF and MALDI-TOF MS

J 2017

Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS

HORKA, M., K. SLAIS, J. SALPLACHTA a Filip RŮŽIČKA

Základní údaje

Originální název

Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS

Autoři

HORKA, M. (203 Česká republika), K. SLAIS (203 Česká republika), J. SALPLACHTA (203 Česká republika) a Filip RŮŽIČKA (203 Česká republika, garant, domácí)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2017, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.123

Kód RIV

RIV/00216224:14110/17:00094480

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2017.08.046

UT WoS

000412671400017

Klíčová slova anglicky

Preparative isoelectric focusing; Colored microorganisms; Isoelectric points; CIEF and MALDI-TOF MS

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 14:13, Soňa Böhmová

Anotace

V originále

Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers. Segments of the separation medium with cells were harvested by a spatula, simply purified using centrifugation and analyzed by capillary isoelectric focusing and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The determined recovery ranged from 78% to 93%. The viability of the harvested cells was verified by their cultivation.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, projekt VaV

Název: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Akronym: VMIMRDIBT)

Investor: Ministerstvo vnitra ČR, Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů

Citovat

HORKA, M., K. SLAIS, J. SALPLACHTA a Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2017, roč. 990, OCT 16 2017, s. 185-193. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2017.08.046.

@article{1395637,
   author = {Horka, M. and Slais, K. and Salplachta, J. and Růžička, Filip},
   article_location = {Amsterdam},
   article_number = {OCT 16 2017},
   doi = {http://dx.doi.org/10.1016/j.aca.2017.08.046},
   keywords = {Preparative isoelectric focusing; Colored microorganisms; Isoelectric points; CIEF and MALDI-TOF MS},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS},
   volume = {990},
   year = {2017}
}

TY  - JOUR
ID  - 1395637
AU  - Horka, M. - Slais, K. - Salplachta, J. - Růžička, Filip
PY  - 2017
TI  - Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS
JF  - Analytica Chimica Acta
VL  - 990
IS  - OCT 16 2017
SP  - 185-193
EP  - 185-193
PB  - Elsevier Science publishers
SN  - 00032670
KW  - Preparative isoelectric focusing
KW  - Colored microorganisms
KW  - Isoelectric points
KW  - CIEF and MALDI-TOF MS
N2  - Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers. Segments of the separation medium with cells were harvested by a spatula, simply purified using centrifugation and analyzed by capillary isoelectric focusing and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The determined recovery ranged from 78% to 93%. The viability of the harvested cells was verified by their cultivation.
ER  -

HORKA, M., K. SLAIS, J. SALPLACHTA a Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2017, roč.~990, OCT 16 2017, s.~185-193. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2017.08.046.

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