Association of matrix metalloproteinases gene variants with
recurrent aphthous stomatitis

a 2017

Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis

SLEZÁKOVÁ, Simona, Petra BOŘILOVÁ LINHARTOVÁ, Jiřina BÁRTOVÁ, Jitka PETANOVÁ, Pavel KUKLÍNEK et. al.

Základní údaje

Originální název

Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis

Autoři

SLEZÁKOVÁ, Simona (203 Česká republika, domácí), Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, domácí), Jiřina BÁRTOVÁ (203 Česká republika), Jitka PETANOVÁ (203 Česká republika), Pavel KUKLÍNEK (203 Česká republika, domácí), Antonín FASSMANN (203 Česká republika, domácí) a Lydie IZAKOVIČOVÁ HOLLÁ (203 Česká republika, domácí)

Vydání

XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30208 Dentistry, oral surgery and medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/17:00100753

Organizační jednotka

Lékařská fakulta

ISBN

978-80-210-8765-1

Klíčová slova anglicky

matrix metalloproteinase; association studies; polymorphism; recurrent aphthous stomatitis

Změněno: 18. 1. 2019 14:08, Mgr. et Mgr. Simona Slezáková, Ph.D.

Anotace

V originále

Background: Recurrent aphthous stomatitis (RAS) is a common inflammatory disease of oral mucosa manifested by erosions and ulcers. Variability in genes coding matrix metalloproteinases (MMPs), the proteolytic enzymes modulating inflammation, has been previously associated with RAS. The aim of our study was to investigate six single nucleotide polymorphisms (SNPs) in genes for gelatinases (MMP2, MMP9) and stromelysin (MMP3) in patients with RAS and healthy controls. Subjects and methods: Totally, 226 subjects were enrolled in this case-control study: 71 patients with RAS and 155 healthy individuals were genotyped for SNPs in the MMP2 (rs243865, rs2287074), MMP3 (rs679620, rs35068180) and MMP9 (rs3918242, rs17576) genes by TaqMan qPCR or by PCR with restriction analysis. Results: While allele frequencies of rs3918242 MMP9 polymorphism and haplotype MMP9 (rs3918242, rs17576) were marginally significantly different between patients with RAS and healthy controls (P=0.04 and P=0.06, respectively), distribution of allele, genotype and haplotype frequencies of the other studied SNPs were found similar in both groups. Nevertheless, the period of ulcer healing significantly less frequently exceeded one week in the MMP3 (rs35068180) 6A/6A carriers and MMP3 (rs679620) CC carriers compared to other genotypes (P=0.01 for both). Conclusions: Our study suggests that MMP9 gene variability, affecting MMP9 expression and activity, may be involved in the development of RAS in the Czech population. Moreover, MMP3 variants could influence the duration of ulcer healing.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/0948/2016, interní kód MU

Název: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba

Investor: Masarykova univerzita, Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV15-29336A, projekt VaV

Název: Změny imunologické reaktivity u pacientů s recidivujícími aftami

Citovat

SLEZÁKOVÁ, Simona, Petra BOŘILOVÁ LINHARTOVÁ, Jiřina BÁRTOVÁ, Jitka PETANOVÁ, Pavel KUKLÍNEK, Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis. In XVIII. Setkání biochemiků a molekulárních biologů, Brno. 2017. ISBN 978-80-210-8765-1.

@proceedings{1395684,
   author = {Slezáková, Simona and Bořilová Linhartová, Petra and Bártová, Jiřina and Petanová, Jitka and Kuklínek, Pavel and Fassmann, Antonín and Izakovičová Hollá, Lydie},
   booktitle = {XVIII. Setkání biochemiků a molekulárních biologů, Brno},
   keywords = {matrix metalloproteinase; association studies; polymorphism; recurrent aphthous stomatitis},
   language = {eng},
   isbn = {978-80-210-8765-1},
   title = {Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis},
   year = {2017}
}

TY  - CONF
ID  - 1395684
AU  - Slezáková, Simona - Bořilová Linhartová, Petra - Bártová, Jiřina - Petanová, Jitka - Kuklínek, Pavel - Fassmann, Antonín - Izakovičová Hollá, Lydie
PY  - 2017
TI  - Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis
SN  - 9788021087651
KW  - matrix metalloproteinase
KW  - association studies
KW  - polymorphism
KW  - recurrent aphthous stomatitis
N2  - Background: Recurrent aphthous stomatitis (RAS) is a common inflammatory disease of oral mucosa manifested by erosions and ulcers. Variability in genes coding matrix metalloproteinases (MMPs), the proteolytic enzymes modulating inflammation, has been previously associated with RAS. The aim of our study was to investigate six single nucleotide polymorphisms (SNPs) in genes for gelatinases (MMP2, MMP9) and stromelysin (MMP3) in patients with RAS and healthy controls. Subjects and methods: Totally, 226 subjects were enrolled in this case-control study: 71 patients with RAS and 155 healthy individuals were genotyped for SNPs in the MMP2 (rs243865, rs2287074), MMP3 (rs679620, rs35068180) and MMP9 (rs3918242, rs17576) genes by TaqMan qPCR or by PCR with restriction analysis. Results: While allele frequencies of rs3918242 MMP9 polymorphism and haplotype MMP9 (rs3918242, rs17576) were marginally significantly different between patients with RAS and healthy controls (P=0.04 and P=0.06, respectively), distribution of allele, genotype and haplotype frequencies of the other studied SNPs were found similar in both groups. Nevertheless, the period of ulcer healing significantly less frequently exceeded one week in the MMP3 (rs35068180) 6A/6A carriers and MMP3 (rs679620) CC carriers compared to other genotypes (P=0.01 for both). Conclusions: Our study suggests that MMP9 gene variability, affecting MMP9 expression and activity, may be involved in the development of RAS in the Czech population. Moreover, MMP3 variants could influence the duration of ulcer healing.
ER  -

SLEZÁKOVÁ, Simona, Petra BOŘILOVÁ LINHARTOVÁ, Jiřina BÁRTOVÁ, Jitka PETANOVÁ, Pavel KUKLÍNEK, Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis. In \textit{XVIII. Setkání biochemiků a molekulárních biologů, Brno}. 2017. ISBN~978-80-210-8765-1.

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