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@article{1396924,
author = {Meader, Ellie and Bárta, Tomáš and MelguizoandSanchis, Dario and Tilgner, Katarzyna and Montaner, David and ElandHarouni, Ashraf A. and Armstrong, Lyle and Lako, Majlinda},
article_location = {Hoboken},
article_number = {1},
doi = {http://dx.doi.org/10.1002/stem.2724},
keywords = {Human embryonic stem cells; miRNAs; Epithelial-mesenchymal transition; Hematopoietic differentiation},
language = {eng},
issn = {1066-5099},
journal = {Stem Cells},
title = {Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs},
url = {http://onlinelibrary.wiley.com/doi/10.1002/stem.2724/full},
volume = {36},
year = {2018}
}
TY - JOUR
ID - 1396924
AU - Meader, Ellie - Bárta, Tomáš - Melguizo-Sanchis, Dario - Tilgner, Katarzyna - Montaner, David - El-Harouni, Ashraf A. - Armstrong, Lyle - Lako, Majlinda
PY - 2018
TI - Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs
JF - Stem Cells
VL - 36
IS - 1
SP - 55-64
EP - 55-64
PB - Wiley-Blackwell
SN - 10665099
KW - Human embryonic stem cells
KW - miRNAs
KW - Epithelial-mesenchymal transition
KW - Hematopoietic differentiation
UR - http://onlinelibrary.wiley.com/doi/10.1002/stem.2724/full
N2 - Hematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression and are responsible for their failure to engraft. We compared the miRNA expression profile of hematopoietic progenitors derived from pluripotent stem cells to those derived from bone marrow and found that numerous miRNAs are too highly expressed in hematopoietic progenitors derived from pluripotent stem cells, and that most of these are inhibitors of epithelial-mesenchymal transition or metastasis (including miR-200b, miR-200c, miR-205, miR-148a, and miR-424). We hypothesize that the high expression of these factors, which promote an adherent phenotype, may be causing the defect in hematopoietic differentiation. However, inhibiting these miRNAs, individually or in multiplex, was insufficient to improve hematopoietic differentiation in vitro, suggesting that other miRNAs and/or genes may be involved in this process.
ER -
MEADER, Ellie, Tomáš BÁRTA, Dario MELGUIZO-SANCHIS, Katarzyna TILGNER, David MONTANER, Ashraf A. EL-HAROUNI, Lyle ARMSTRONG a Majlinda LAKO. Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, roč.~36, č.~1, s.~55-64. ISSN~1066-5099. doi:10.1002/stem.2724. 2018.
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