Photon-Upconversion Nanoparticles Give a New Twist to Classic
Analytical Tools

a 2017

Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools

GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA a Antonín HLAVÁČEK

Základní údaje

Originální název

Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools

Autoři

GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA a Antonín HLAVÁČEK

Vydání

2017 MRS Fall Meeting & Exhibit, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

photon-upconversion nanoparticle; electrophoretic separation; single-molecule analysis; upconversion-linked immunosorbent assay

Příznaky

Mezinárodní význam

Změněno: 24. 2. 2019 17:33, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Conventional fluorescence spectroscopy is limited by autofluorescence and light scattering of the surrounding matrix. This optical background interference can be elegantly avoided by using photon-upconversion nanoparticles (UCNPs) that emit short-wavelength light under near-infrared (NIR, 980 nm) excitation (anti-Stokes emission). I will describe new nano-analytical techniques taking full advantage of UCNPs. Agarose gel electrophoresis enables the separation and purification of UCNPs that can then be detected in the gel by a 980-nm laser scanner. The purifiation of UCNPs bioconjugates has the potential to improve many bioanalytical applications. Such well-defined UCNPs can be used to replace conventional enzyme-mediated amplification systems in microtiter plate immunoassays. For example, an upconversion-linked immunoassay (ULISA) allowed for the sensitive detection of the pharmaceutical diclofenac in environmental water samples. UCNPs can be detected at the single nanoparticle level using a relatively simple wide-field upconversion microscope. In this way, it is possible to implement a single molecule sandwich immunoassay for the detection of diagnostic markers such as prostate-specific antigen (PSA).

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA a Antonín HLAVÁČEK. Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools. In 2017 MRS Fall Meeting & Exhibit. 2017.

@proceedings{1398200,
   author = {Gorris, HansandHeiner and Mickert, Matthias Jürgen and Farka, Zdeněk and Hlaváček, Antonín},
   booktitle = {2017 MRS Fall Meeting & Exhibit},
   keywords = {photon-upconversion nanoparticle; electrophoretic separation; single-molecule analysis; upconversion-linked immunosorbent assay},
   language = {eng},
   title = {Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools},
   url = {http://www.mrs.org/fall2017-symposium-sessions?code=NM07},
   year = {2017}
}

TY  - CONF
ID  - 1398200
AU  - Gorris, Hans-Heiner - Mickert, Matthias Jürgen - Farka, Zdeněk - Hlaváček, Antonín
PY  - 2017
TI  - Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools
KW  - photon-upconversion nanoparticle
KW  - electrophoretic separation
KW  - single-molecule analysis
KW  - upconversion-linked immunosorbent assay
UR  - http://www.mrs.org/fall2017-symposium-sessions?code=NM07
N2  - Conventional fluorescence spectroscopy is limited by autofluorescence and light scattering of the surrounding matrix. This optical background interference can be elegantly avoided by using photon-upconversion nanoparticles (UCNPs) that emit short-wavelength light under near-infrared (NIR, 980 nm) excitation (anti-Stokes emission). I will describe new nano-analytical techniques taking full advantage of UCNPs. Agarose gel electrophoresis enables the separation and purification of UCNPs that can then be detected in the gel by a 980-nm laser scanner. The purifiation of UCNPs bioconjugates has the potential to improve many bioanalytical applications. Such well-defined UCNPs can be used to replace conventional enzyme-mediated amplification systems in microtiter plate immunoassays. For example, an upconversion-linked immunoassay (ULISA) allowed for the sensitive detection of the pharmaceutical diclofenac in environmental water samples. UCNPs can be detected at the single nanoparticle level using a relatively simple wide-field upconversion microscope. In this way, it is possible to implement a single molecule sandwich immunoassay for the detection of diagnostic markers such as prostate-specific antigen (PSA).
ER  -

GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA a Antonín HLAVÁČEK. Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools. In \textit{2017 MRS Fall Meeting \&{} Exhibit}. 2017.

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