MALDI MSI of MeLiM melanoma: Searching for differences in
protein profiles

J 2017

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

GURÁŇ, Roman; Lucie VANICKOVA; Vratislav HORAK; Soňa KŘÍŽKOVÁ; Petr MICHÁLEK et al.

Základní údaje

Originální název

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

Autoři

GURÁŇ, Roman; Lucie VANICKOVA; Vratislav HORAK; Soňa KŘÍŽKOVÁ; Petr MICHÁLEK; Zbynek HEGER; Ondřej ZÍTKA a Vojtěch ADAM

Vydání

PLOS ONE, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Označené pro přenos do RIV

DOI

https://doi.org/10.1371/journal.pone.0189305

Klíčová slova anglicky

MALDI; Mass spectrometry imaging; minipig; melanoma; proteins

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 12. 2017 13:20, Mgr. Roman Guráň

Anotace

V originále

Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and clinical parameters, and the identification of additional tumor markers is thus of paramount importance. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI), an important tool in cancer research, is useful for unraveling the molecular profile of melanoma. Methodology/Principal findings In this report, we used the melanoma-bearing Libechov minipig (MeLiM), a unique animal model that allows observation of the complete spontaneous regression of invasive cutaneous melanoma, to investigate i) the differences between melanoma and healthy skin protein profiles and ii) the proteins potentially involved in spontaneous regression. The MeLiM tissues were cryosected, histologically characterized, analyzed by MALDI MSI, and immunohistologically stained. Multivariate statistical analyses of the MALDI MSI data revealed ten relevant m/z ions, of which the expression levels varied significantly among the studied MeLiM tissues. These ion peaks were used to create mass ion images/maps and visualize the differences between tumor and healthy skin specimens, as well as among histologically characterized tissue regions. Conclusions/Significance Protein profiles comprising ten statistically significant mass ion peaks useful for differentiating cutaneous melanoma and healthy skin tissues were determined. Peaks at m/z 3044, 6011, 6140 and 10180 were overexpressed in melanoma compared with healthy skin tissue. More specifically, m/z 6140 was expressed at significantly (p < 0.05) higher levels in normally growing melanoma regions than in regions with early and late spontaneous regression. This study demonstrates the clinical utility of MALDI MSI for the analysis of tissue cryosections at a molecular level.

Citovat

GURÁŇ, Roman; Lucie VANICKOVA; Vratislav HORAK; Soňa KŘÍŽKOVÁ; Petr MICHÁLEK; Zbynek HEGER; Ondřej ZÍTKA a Vojtěch ADAM. MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles. PLOS ONE. Public Library of Science, 2017. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0189305.

@article{1398955,
   author = {Guráň, Roman and Vanickova, Lucie and Horak, Vratislav and Křížková, Soňa and Michálek, Petr and Heger, Zbynek and Zítka, Ondřej and Adam, Vojtěch},
   doi = {https://doi.org/10.1371/journal.pone.0189305},
   keywords = {MALDI; Mass spectrometry imaging; minipig; melanoma; proteins},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles},
   url = {https://doi.org/10.1371/journal.pone.0189305},
   year = {2017}
}

TY  - JOUR
ID  - 1398955
AU  - Guráň, Roman - Vanickova, Lucie - Horak, Vratislav - Křížková, Soňa - Michálek, Petr - Heger, Zbynek - Zítka, Ondřej - Adam, Vojtěch
PY  - 2017
TI  - MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles
JF  - PLOS ONE
PB  - Public Library of Science
SN  - 19326203
KW  - MALDI
KW  - Mass spectrometry imaging
KW  - minipig
KW  - melanoma
KW  - proteins
UR  - https://doi.org/10.1371/journal.pone.0189305
N2  - Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and clinical parameters, and the identification of additional tumor markers is thus of paramount importance. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI), an important tool in cancer research, is useful for unraveling the molecular profile of melanoma. Methodology/Principal findings In this report, we used the melanoma-bearing Libechov minipig (MeLiM), a unique animal model that allows observation of the complete spontaneous regression of invasive cutaneous melanoma, to investigate i) the differences between melanoma and healthy skin protein profiles and ii) the proteins potentially involved in spontaneous regression. The MeLiM tissues were cryosected, histologically characterized, analyzed by MALDI MSI, and immunohistologically stained. Multivariate statistical analyses of the MALDI MSI data revealed ten relevant m/z ions, of which the expression levels varied significantly among the studied MeLiM tissues. These ion peaks were used to create mass ion images/maps and visualize the differences between tumor and healthy skin specimens, as well as among histologically characterized tissue regions. Conclusions/Significance Protein profiles comprising ten statistically significant mass ion peaks useful for differentiating cutaneous melanoma and healthy skin tissues were determined. Peaks at m/z 3044, 6011, 6140 and 10180 were overexpressed in melanoma compared with healthy skin tissue. More specifically, m/z 6140 was expressed at significantly (p < 0.05) higher levels in normally growing melanoma regions than in regions with early and late spontaneous regression. This study demonstrates the clinical utility of MALDI MSI for the analysis of tissue cryosections at a molecular level.
ER  -

GURÁŇ, Roman; Lucie VANICKOVA; Vratislav HORAK; Soňa KŘÍŽKOVÁ; Petr MICHÁLEK; Zbynek HEGER; Ondřej ZÍTKA a Vojtěch ADAM. MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles. \textit{PLOS ONE}. Public Library of Science, 2017. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0189305.

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