Carboxyl-rich plasma polymer surfaces in surface plasmon
resonance immunosensing

J 2018

Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

MAKHNEVA, Ekaterina; Adam OBRUSNÍK; Zdeněk FARKA; Petr SKLÁDAL; Marianne VANDENBOSSCHE et al.

Základní údaje

Originální název

Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Autoři

MAKHNEVA, Ekaterina; Adam OBRUSNÍK; Zdeněk FARKA; Petr SKLÁDAL; Marianne VANDENBOSSCHE; Dirk HEGEMANN a Lenka ZAJÍČKOVÁ

Vydání

Japanese Journal of Applied Physics, Bristol, IOP Publishing, 2018, 0021-4922

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10305 Fluids and plasma physics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.471

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00102139

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

carboxyl-rich plasma polymer; immobilization; surface plasmon resonance; immunosensor

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 13:02, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MAKHNEVA, Ekaterina; Adam OBRUSNÍK; Zdeněk FARKA; Petr SKLÁDAL; Marianne VANDENBOSSCHE; Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing. Japanese Journal of Applied Physics. Bristol: IOP Publishing, 2018, roč. 57, 01AG06, s. "01AG06-1"-"01AG06-5", 5 s. ISSN 0021-4922. Dostupné z: https://doi.org/10.7567/JJAP.57.01AG06.

@article{1399998,
   author = {Makhneva, Ekaterina and Obrusník, Adam and Farka, Zdeněk and Skládal, Petr and Vandenbossche, Marianne and Hegemann, Dirk and Zajíčková, Lenka},
   article_location = {Bristol},
   article_number = {01AG06},
   doi = {https://doi.org/10.7567/JJAP.57.01AG06},
   keywords = {carboxyl-rich plasma polymer; immobilization; surface plasmon resonance; immunosensor},
   language = {eng},
   issn = {0021-4922},
   journal = {Japanese Journal of Applied Physics},
   title = {Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing},
   volume = {57},
   year = {2018}
}

TY  - JOUR
ID  - 1399998
AU  - Makhneva, Ekaterina - Obrusník, Adam - Farka, Zdeněk - Skládal, Petr - Vandenbossche, Marianne - Hegemann, Dirk - Zajíčková, Lenka
PY  - 2018
TI  - Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing
JF  - Japanese Journal of Applied Physics
VL  - 57
IS  - 01AG06
SP  - "01AG06-1"-"01AG06-5"
EP  - "01AG06-1"-"01AG06-5"
PB  - IOP Publishing
SN  - 00214922
KW  - carboxyl-rich plasma polymer
KW  - immobilization
KW  - surface plasmon resonance
KW  - immunosensor
N2  - Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.
ER  -

MAKHNEVA, Ekaterina; Adam OBRUSNÍK; Zdeněk FARKA; Petr SKLÁDAL; Marianne VANDENBOSSCHE; Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing. \textit{Japanese Journal of Applied Physics}. Bristol: IOP Publishing, 2018, roč.~57, 01AG06, s.~''01AG06-1''-''01AG06-5'', 5 s. ISSN~0021-4922. Dostupné z: https://doi.org/10.7567/JJAP.57.01AG06.

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