| MAKHNEVA, Ekaterina, Adam OBRUSNÍK, Zdeněk FARKA, Petr SKLÁDAL, Marianne VANDENBOSSCHE, Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing. Japanese Journal of Applied Physics. Bristol: IOP Publishing, 2018, roč. 57, 01AG06, s. "01AG06-1"-"01AG06-5", 5 s. ISSN 0021-4922. Dostupné z: https://dx.doi.org/10.7567/JJAP.57.01AG06. |
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@article{1399998,
author = {Makhneva, Ekaterina and Obrusník, Adam and Farka, Zdeněk and Skládal, Petr and Vandenbossche, Marianne and Hegemann, Dirk and Zajíčková, Lenka},
article_location = {Bristol},
article_number = {01AG06},
doi = {http://dx.doi.org/10.7567/JJAP.57.01AG06},
keywords = {carboxyl-rich plasma polymer; immobilization; surface plasmon resonance; immunosensor},
language = {eng},
issn = {0021-4922},
journal = {Japanese Journal of Applied Physics},
title = {Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing},
volume = {57},
year = {2018}
}
TY - JOUR
ID - 1399998
AU - Makhneva, Ekaterina - Obrusník, Adam - Farka, Zdeněk - Skládal, Petr - Vandenbossche, Marianne - Hegemann, Dirk - Zajíčková, Lenka
PY - 2018
TI - Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing
JF - Japanese Journal of Applied Physics
VL - 57
IS - 01AG06
SP - "01AG06-1"-"01AG06-5"
EP - "01AG06-1"-"01AG06-5"
PB - IOP Publishing
SN - 00214922
KW - carboxyl-rich plasma polymer
KW - immobilization
KW - surface plasmon resonance
KW - immunosensor
N2 - Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.
ER -
MAKHNEVA, Ekaterina, Adam OBRUSNÍK, Zdeněk FARKA, Petr SKLÁDAL, Marianne VANDENBOSSCHE, Dirk HEGEMANN a Lenka ZAJÍČKOVÁ. Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing. \textit{Japanese Journal of Applied Physics}. Bristol: IOP Publishing, 2018, roč.~57, 01AG06, s.~''01AG06-1''-''01AG06-5'', 5 s. ISSN~0021-4922. Dostupné z: https://dx.doi.org/10.7567/JJAP.57.01AG06.
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