| RAMANA, Pranov, Jan SCHEJBAL, Kristof HOUTHOOFD, Johan MARTENS, Adams ERWIN, Patrick AUGUSTIJNS, Zdeněk GLATZ a Ann VAN SCHEPDAEL. An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors. Online. Electrophoresis. Německo: WILEY-VCH Verlag GmbH, 2018, roč. 2018, č. 7, s. 981-988. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201700434. [citováno 2024-04-24] |
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@article{1400918,
author = {Ramana, Pranov and Schejbal, Jan and Houthoofd, Kristof and Martens, Johan and Erwin, Adams and Augustijns, Patrick and Glatz, Zdeněk and Van Schepdael, Ann},
article_location = {Německo},
article_number = {7},
doi = {http://dx.doi.org/10.1002/elps.201700434},
keywords = {IMER; Magnetic microparticles; Immobilization; In-line capillary electrophoresis},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors},
url = {http://dx.doi.org/10.1002/elps.201700434},
volume = {2018},
year = {2018}
}
TY - JOUR
ID - 1400918
AU - Ramana, Pranov - Schejbal, Jan - Houthoofd, Kristof - Martens, Johan - Erwin, Adams - Augustijns, Patrick - Glatz, Zdeněk - Van Schepdael, Ann
PY - 2018
TI - An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors
JF - Electrophoresis
VL - 2018
IS - 7
SP - 981-988
EP - 981-988
PB - WILEY-VCH Verlag GmbH
SN - 01730835
KW - IMER
KW - Magnetic microparticles
KW - Immobilization
KW - In-line capillary electrophoresis
UR - http://dx.doi.org/10.1002/elps.201700434
N2 - In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate.
ER -
RAMANA, Pranov, Jan SCHEJBAL, Kristof HOUTHOOFD, Johan MARTENS, Adams ERWIN, Patrick AUGUSTIJNS, Zdeněk GLATZ a Ann VAN SCHEPDAEL. An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors. Online. \textit{Electrophoresis}. Německo: WILEY-VCH Verlag GmbH, 2018, roč.~2018, č.~7, s.~981-988. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201700434. [citováno 2024-04-24]
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