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@article{1401134,
author = {Brabencová, Sylva and Ihnatová, Ivana and Potěšil, David and Fojtová, Miloslava and Fajkus, Jiří and Zdráhal, Zbyněk and Lochmanová, Gabriela},
article_number = {2084},
doi = {http://dx.doi.org/10.3389/fpls.2017.02084},
keywords = {Arabidopsis thaliana; ecotype; histone; post-translational modifications; epigenetics; mass spectrometry},
language = {eng},
issn = {1664-462X},
journal = {Frontiers in Plant Science},
title = {Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors},
volume = {8},
year = {2017}
}
TY - JOUR
ID - 1401134
AU - Brabencová, Sylva - Ihnatová, Ivana - Potěšil, David - Fojtová, Miloslava - Fajkus, Jiří - Zdráhal, Zbyněk - Lochmanová, Gabriela
PY - 2017
TI - Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors
JF - Frontiers in Plant Science
VL - 8
IS - 2084
SP - 1-12
EP - 1-12
PB - Frontiers Research Foundation
SN - 1664462X
KW - Arabidopsis thaliana
KW - ecotype
KW - histone
KW - post-translational modifications
KW - epigenetics
KW - mass spectrometry
N2 - Inter-individual variability of conspecific plants is governed by differences in their genetically determined growth and development traits, environmental conditions, and adaptive responses under epigenetic control involving histone post-translational modifications. The apparent variability in histone modifications among plants might be increased by technical variation introduced in sample processing during epigenetic analyses. Thus, to detect true variations in epigenetic histone patterns associated with given factors, the basal variability among samples that is not associated with them must be estimated. To improve knowledge of relative contribution of biological and technical variation, mass spectrometry was used to examine histone modification patterns (acetylation and methylation) among Arabidopsis thaliana plants of ecotypes Columbia 0 (Col-0) and Wassilewskija (Ws) homogenized by two techniques (grinding in a cryomill or with a mortar and pestle). We found little difference in histone modification profiles between the ecotypes. However, in comparison of the biological and technical components of variability, we found consistently higher inter-individual variability in histone mark levels among Ws plants than among Col-0 plants (grown from seeds collected either from single plants or sets of plants). Thus, more replicates of Ws would be needed for rigorous analysis of epigenetic marks. Regarding technical variability, the cryomill introduced detectably more heterogeneity in the data than the mortar and pestle treatment, but mass spectrometric analyses had minor apparent effects. Our study shows that it is essential to consider inter-sample variance and estimate suitable numbers of biological replicates for statistical analysis for each studied organism when investigating changes in epigenetic histone profiles.
ER -
BRABENCOVÁ, Sylva, Ivana IHNATOVÁ, David POTĚŠIL, Miloslava FOJTOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL a Gabriela LOCHMANOVÁ. Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors. \textit{Frontiers in Plant Science}. Frontiers Research Foundation, 2017, roč.~8, č.~2084, s.~1-12. ISSN~1664-462X. Dostupné z: https://dx.doi.org/10.3389/fpls.2017.02084.
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