Liquid chromatography with APCI and SALDI mass spectrometry
detection using silver nanoparticles in cholesteryl esters
analysis

a 2017

Liquid chromatography with APCI and SALDI mass spectrometry detection using silver nanoparticles in cholesteryl esters analysis

ROBLOVÁ, Vendula, Miroslav LÍSA a Jan PREISLER

Základní údaje

Originální název

Liquid chromatography with APCI and SALDI mass spectrometry detection using silver nanoparticles in cholesteryl esters analysis

Autoři

ROBLOVÁ, Vendula (203 Česká republika, domácí), Miroslav LÍSA (203 Česká republika) a Jan PREISLER (203 Česká republika, garant, domácí)

Vydání

HPLC 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/17:00095341

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

lipids; cholesteryl esters; silver nanoparticles; SALDI; APCI; mass spectrometry

Změněno: 9. 1. 2018 13:28, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

Nonpolar lipid separation can be accomplished by several chromatographic modes according to the length of the fatty acyl chains and the number of double bonds position. Reversed-phase high performance liquid chromatography (RP-HPLC) in combination with UV detection at low wavelengths is often used for the detection of common lipids, but low sensitivity may cause difficulties. In this case, HPLC coupled to mass spectrometry with atmospheric pressure chemical ionization (APCI) can provide more specific information about studied analytes. Presented work is aimed to the combination of RP-HPLC with on-line UV and off-line mass spectrometry detection for cholesteryl esters determination. A simple HPLC method with on-line UV and APCI MS detection was proposed for analysis of cholesteryl esters with different number of double bonds in fatty acids chain in our case were selected cholesteryl ester stearate (CHS), cholesteryl ester oleate (CHE), cholesteryl ester linoleate (CHL) and cholesteryl ester arachidonate (CHA) [1]. Off-line detection was facilitated by surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) using suspension of silver nanoparticles as matrix. After separation, a splitter combined with a laboratory-built spotter was used for eluent deposition on a target over a layer of silver nanoparticles for SALDI experiments [2]. The selected analytes were characterized based on comparison of identified fragment ions from spectra obtained by those two techniques. In SALDI spectra, cholesteryl esters fragment ions characteristic for fatty acids or molecular adduct ions with silver and sodium were observed. On the other hand, spectra obtained by APCI MS showed only cholesterol (Ch) fragment ion [Ch-H2O+H]+ (m/z 369.35). Combination of MS techniques with different ionization mechanisms allow obtaining complementary information for better characterization of the studied lipids. Developed method was used for determination of presented cholesteryl esters in human plasma samples.

Návaznosti

GA15-05387S, projekt VaV

Název: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Grantová agentura ČR, Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ROBLOVÁ, Vendula, Miroslav LÍSA a Jan PREISLER. Liquid chromatography with APCI and SALDI mass spectrometry detection using silver nanoparticles in cholesteryl esters analysis. In HPLC 2017. 2017.

@proceedings{1401946,
   author = {Roblová, Vendula and Lísa, Miroslav and Preisler, Jan},
   booktitle = {HPLC 2017},
   keywords = {lipids; cholesteryl esters; silver nanoparticles; SALDI; APCI; mass spectrometry},
   language = {eng},
   title = {Liquid chromatography with APCI and SALDI mass spectrometry detection using silver nanoparticles in cholesteryl esters analysis},
   year = {2017}
}

TY  - CONF
ID  - 1401946
AU  - Roblová, Vendula - Lísa, Miroslav - Preisler, Jan
PY  - 2017
TI  - Liquid chromatography with APCI and SALDI mass spectrometry detection using silver nanoparticles in cholesteryl esters analysis
KW  - lipids
KW  - cholesteryl esters
KW  - silver nanoparticles
KW  - SALDI
KW  - APCI
KW  - mass spectrometry
N2  - Nonpolar lipid separation can be accomplished by several chromatographic modes according to the length of the fatty acyl chains and the number of double bonds position. Reversed-phase high performance liquid chromatography (RP-HPLC) in combination with UV detection at low wavelengths is often used for the detection of common lipids, but low sensitivity may cause difficulties. In this case, HPLC coupled to mass spectrometry with atmospheric pressure chemical ionization (APCI) can provide more specific information about studied analytes. Presented work is aimed to the combination of RP-HPLC with on-line UV and off-line mass spectrometry detection for cholesteryl esters determination. A simple HPLC method with on-line UV and APCI MS detection was proposed for analysis of cholesteryl esters with different number of double bonds in fatty acids chain in our case were selected cholesteryl ester stearate (CHS), cholesteryl ester oleate (CHE), cholesteryl ester linoleate (CHL) and cholesteryl ester arachidonate (CHA) [1]. Off-line detection was facilitated by surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) using suspension of silver nanoparticles as matrix. After separation, a splitter combined with a laboratory-built spotter was used for eluent deposition on a target over a layer of silver nanoparticles for SALDI experiments [2]. The selected analytes were characterized based on comparison of identified fragment ions from spectra obtained by those two techniques. In SALDI spectra, cholesteryl esters fragment ions characteristic for fatty acids or molecular adduct ions with silver and sodium were observed. On the other hand, spectra obtained by APCI MS showed only cholesterol (Ch) fragment ion [Ch-H2O+H]+ (m/z 369.35). Combination of MS techniques with different ionization mechanisms allow obtaining complementary information for better characterization of the studied lipids. Developed method was used for determination of presented cholesteryl esters in human plasma samples.
ER  -

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