KNAPP, Michal, Pavel DOBEŠ, Michal ŘEŘICHA a Pavel HYRŠL. Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods. European Journal of Entomology, AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, roč. 115, č. 1, s. 1-6. ISSN 1802-8829. doi:10.14411/eje.2018.001.
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Základní údaje
Originální název Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Název česky Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Autoři KNAPP, Michal (203 Česká republika), Pavel DOBEŠ (203 Česká republika, garant, domácí), Michal ŘEŘICHA (203 Česká republika) a Pavel HYRŠL (203 Česká republika, domácí).
Vydání European Journal of Entomology, AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, 1802-8829.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor Immunology
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 1.017 v roce 2017
Kód RIV RIV/00216224:14310/18:00102177
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.14411/eje.2018.001
UT WoS 000434150700001
Klíčová slova česky Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invazivní druh; hemolymfa; antimikrobiální aktivita; koncentrace hemocytů; koncenttrace proteinů; reflexní krvácení; metoda vzorkování
Klíčová slova anglicky Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invasive species; haemolymph; antimicrobial activity; haemocyte concentration; protein concentration; reflex bleeding; sampling technique
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: Mgr. Pavel Dobeš, Ph.D., učo 150960. Změněno: 14. 10. 2018 15:24.
Anotace
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.
Anotace česky
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.
VytisknoutZobrazeno: 19. 8. 2019 20:48