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@article{1402139, author = {Knapp, Michal and Dobeš, Pavel and Řeřicha, Michal and Hyršl, Pavel}, article_number = {1}, doi = {http://dx.doi.org/10.14411/eje.2018.001}, keywords = {Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invasive species; haemolymph; antimicrobial activity; haemocyte concentration; protein concentration; reflex bleeding; sampling technique}, language = {eng}, issn = {1802-8829}, journal = {European Journal of Entomology}, title = {Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods}, url = {https://www.eje.cz/artkey/eje-201801-0001_Puncture_vs_reflex_bleeding_Haemolymph_composition_reveals_significant_differences_among_ladybird_species_Co.php}, volume = {115}, year = {2018} }
TY - JOUR ID - 1402139 AU - Knapp, Michal - Dobeš, Pavel - Řeřicha, Michal - Hyršl, Pavel PY - 2018 TI - Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods JF - European Journal of Entomology VL - 115 IS - 1 SP - 1-6 EP - 1-6 PB - AV ČR, Institute of Entomology of the Czech Academy of Sciences SN - 18028829 KW - Coleoptera KW - Coccinellidae KW - Harmonia axyridis KW - Coccinella septempunctata KW - Ceratomegilla undecimnotata KW - invasive species KW - haemolymph KW - antimicrobial activity KW - haemocyte concentration KW - protein concentration KW - reflex bleeding KW - sampling technique UR - https://www.eje.cz/artkey/eje-201801-0001_Puncture_vs_reflex_bleeding_Haemolymph_composition_reveals_significant_differences_among_ladybird_species_Co.php N2 - Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli. ER -
KNAPP, Michal, Pavel DOBEŠ, Michal ŘEŘICHA a Pavel HYRŠL. Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods. \textit{European Journal of Entomology}. AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, roč.~115, č.~1, s.~1-6. ISSN~1802-8829. Dostupné z: https://dx.doi.org/10.14411/eje.2018.001.
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