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@article{1403082,
author = {Kranc, W. and Brazert, M. and Ozegowska, K. and Nawrocki, M. J. and Budna, J. and Celichowski, P. and DyszkiewiczandKonwinska, M. and Jankowski, M. and Ješeta, Michal and Pawelczyk, L. and Bruska, M. and Nowicki, M. and Zabel, M. and Kempisty, B.},
article_location = {Basel},
article_number = {12},
doi = {http://dx.doi.org/10.3390/ijms18122673},
keywords = {human; granulosa cells; in vitro culture (IVC); steroid biosynthesis},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach},
url = {http://dx.doi.org/10.3390/ijms18122673},
volume = {18},
year = {2017}
}
TY - JOUR
ID - 1403082
AU - Kranc, W. - Brazert, M. - Ozegowska, K. - Nawrocki, M. J. - Budna, J. - Celichowski, P. - Dyszkiewicz-Konwinska, M. - Jankowski, M. - Ješeta, Michal - Pawelczyk, L. - Bruska, M. - Nowicki, M. - Zabel, M. - Kempisty, B.
PY - 2017
TI - Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach
JF - International Journal of Molecular Sciences
VL - 18
IS - 12
SP - 1-14
EP - 1-14
PB - MDPI
SN - 14220067
KW - human
KW - granulosa cells
KW - in vitro culture (IVC)
KW - steroid biosynthesis
UR - http://dx.doi.org/10.3390/ijms18122673
L2 - http://dx.doi.org/10.3390/ijms18122673
N2 - Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy. Understanding of the exact molecular mechanisms underlying those processes could not only provide us with deep insight into the regulation of the reproductive cycle, but also create new clinical advantages in detection and treatment of various diseases associated with sex hormone abnormalities. We have used the microarray approach validated by RT-qPCR, to analyze the patterns of gene expression in primary cultures of human granulosa cells at days 1, 7, 15, and 30 of said cultures. We have especially focused on genes belonging to ontology groups associated with steroid biosynthesis and metabolism, namely "Regulation of steroid biosynthesis process" and "Regulation of steroid metabolic process". Eleven genes have been chosen, as they exhibited major change under a culture condition. Out of those, ten genes, namely STAR, SCAP, POR, SREBF1, GFI1, SEC14L2, STARD4, INSIG1, DHCR7, and IL1B, belong to both groups. Patterns of expression of those genes were analyzed, along with brief description of their functions. That analysis helped us achieve a better understanding of the exact molecular processes underlying steroid biosynthesis and metabolism in human granulosa cells.
ER -
KRANC, W., M. BRAZERT, K. OZEGOWSKA, M. J. NAWROCKI, J. BUDNA, P. CELICHOWSKI, M. DYSZKIEWICZ-KONWINSKA, M. JANKOWSKI, Michal JEŠETA, L. PAWELCZYK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY. Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2017, roč.~18, č.~12, s.~1-14. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms18122673.
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