Comparative genome analysis of extremophile Acidithiobacillus
ferrooxidans grown on different substrates

a 2017

Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates

GAJDOŠÍK, Martin; Jiří KUČERA; Jan LOCHMAN; Oldřich JANICZEK; Martin MANDL et al.

Základní údaje

Originální název

Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates

Autoři

GAJDOŠÍK, Martin; Jiří KUČERA; Jan LOCHMAN; Oldřich JANICZEK a Martin MANDL

Vydání

XVIII. Setkání biochemiků a molekulárních biologů, 2017

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

ISBN

978-80-210-8765-1

Změněno: 17. 1. 2018 18:39, Mgr. Jiří Kučera, Ph.D.

Anotace

V originále

Acidithiobacillus spp. are Gram-negative, mesophilic, chemolithotrophic bacteria thriving in extremely acidic environments. Due to their ability to oxidize metal sulfides, they are important members of microbial consortia involved in the metal recycling industry. Acidithiobacillus ferrooxidans strain CCM 4253 was isolated from an acid mine drainage at Zlaté Hory in the Czech Republic. Under laboratory conditions, A. ferrooxidans is typically cultivated on elemental sulfur or ferrous iron. Effective bacterial adaptation to different substrates (such as from elemental sulfur to ferrous iron and vice versa) is essential during metal sulfide oxidation. The Illumina sequencing technology was used to monitor potential DNA mutation providing genetic and phenotypic flexibility in response to energy substrate changes. Indeed, the mobile element ISAfe1 was found to be inserted in the pstC2 gene encoding part of the ABC transporter complex involved in phosphate import. In addition, this protein complex belongs to the protein-protein interacting network of rusticyanin, an important iron oxidation marker. Preliminary results suggest one of the prospective regulations of phenotypic switching at the DNA level by reversible inactivation of the pstC2 gene. Further study will aim to determine under what external conditions it occurs. In the future, this knowledge will be essential to control acid mine drainage formation and to optimize metal recovery.

Návaznosti

MUNI/A/1278/2016, interní kód MU

Název: Podpora biochemického výzkumu v roce 2017

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

GAJDOŠÍK, Martin; Jiří KUČERA; Jan LOCHMAN; Oldřich JANICZEK a Martin MANDL. Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates. In XVIII. Setkání biochemiků a molekulárních biologů. 2017. ISBN 978-80-210-8765-1.

@proceedings{1403149,
   author = {Gajdošík, Martin and Kučera, Jiří and Lochman, Jan and Janiczek, Oldřich and Mandl, Martin},
   booktitle = {XVIII. Setkání biochemiků a molekulárních biologů},
   isbn = {978-80-210-8765-1},
   title = {Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates},
   year = {2017}
}

TY  - CONF
ID  - 1403149
AU  - Gajdošík, Martin - Kučera, Jiří - Lochman, Jan - Janiczek, Oldřich - Mandl, Martin
PY  - 2017
TI  - Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates
SN  - 9788021087651
N2  - Acidithiobacillus spp. are Gram-negative, mesophilic, chemolithotrophic bacteria thriving in extremely acidic environments. Due to their ability to oxidize metal sulfides, they are important members of microbial consortia involved in the metal recycling industry. Acidithiobacillus ferrooxidans strain CCM 4253 was isolated from an acid mine drainage at Zlaté Hory in the Czech Republic. Under laboratory conditions, A. ferrooxidans is typically cultivated on elemental sulfur or ferrous iron. Effective bacterial adaptation to different substrates (such as from elemental sulfur to ferrous iron and vice versa) is essential during metal sulfide oxidation. The Illumina sequencing technology was used to monitor potential DNA mutation providing genetic and phenotypic flexibility in response to energy substrate changes. Indeed, the mobile element ISAfe1 was found to be inserted in the pstC2 gene encoding part of the ABC transporter complex involved in phosphate import. In addition, this protein complex belongs to the protein-protein interacting network of rusticyanin, an important iron oxidation marker. Preliminary results suggest one of the prospective regulations of phenotypic switching at the DNA level by reversible inactivation of the pstC2 gene. Further study will aim to determine under what external conditions it occurs. In the future, this knowledge will be essential to control acid mine drainage formation and to optimize metal recovery.
ER  -

GAJDOŠÍK, Martin; Jiří KUČERA; Jan LOCHMAN; Oldřich JANICZEK a Martin MANDL. Comparative genome analysis of extremophile Acidithiobacillus ferrooxidans grown on different substrates. In \textit{XVIII. Setkání biochemiků a molekulárních biologů}. 2017. ISBN~978-80-210-8765-1.

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