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@inbook{1403855,
author = {Kokkonen, Piia Pauliina and Koudeláková, Táňa and Chaloupková, Radka and Daniel, Lukáš and Prokop, Zbyněk and Damborský, Jiří},
address = {Heidelberg},
booktitle = {HANDBOOK OF HYDROCARBON AND LIPID MICROBIOLOGY SERIES. AEROBIC UTILIZATION OF HYDROCARBONS, OILS, AND LIPIDS},
doi = {http://dx.doi.org/10.1007/978-3-319-39782-5},
editor = {Rojo, Fernando},
keywords = {Biodegradation Hydrocarbons Bacteria Yeasts Alkanes Gasoline Aromatic hydrocarbons Degradation of lipids Fatty acids},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Heidelberg},
isbn = {978-3-319-50419-3},
pages = {1-21},
publisher = {Springer, Cham},
title = {Structure-function Relationships and Engineering of Haloalkane Dehalogenases.},
url = {https://is.muni.cz/auth/publikace/edit?vysledek=79477;akce=edit;id=1403855},
year = {2017}
}
TY - CHAP
ID - 1403855
AU - Kokkonen, Piia Pauliina - Koudeláková, Táňa - Chaloupková, Radka - Daniel, Lukáš - Prokop, Zbyněk - Damborský, Jiří
PY - 2017
TI - Structure-function Relationships and Engineering of Haloalkane Dehalogenases.
VL - neuveden
PB - Springer, Cham
CY - Heidelberg
SN - 9783319504193
KW - Biodegradation Hydrocarbons Bacteria Yeasts Alkanes Gasoline Aromatic hydrocarbons Degradation of lipids Fatty acids
UR - https://is.muni.cz/auth/publikace/edit?vysledek=79477;akce=edit;id=1403855
N2 - The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.
ER -
KOKKONEN, Piia Pauliina, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, Lukáš DANIEL, Zbyněk PROKOP a Jiří DAMBORSKÝ. Structure-function Relationships and Engineering of Haloalkane Dehalogenases. Online. In Rojo, Fernando. \textit{HANDBOOK OF HYDROCARBON AND LIPID MICROBIOLOGY SERIES. AEROBIC UTILIZATION OF HYDROCARBONS, OILS, AND LIPIDS}. Heidelberg: Springer, Cham, 2017, s.~1-21. ISBN~978-3-319-50419-3. Dostupné z: https://dx.doi.org/10.1007/978-3-319-39782-5.
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