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@article{1404252,
author = {Kotásková, Iva and Mališová, Barbora and Obručová, Hana and Holá, Veronika and Peroutková, Tereza and Růžička, Filip and Freiberger, Tomáš},
article_location = {Basel},
article_number = {6},
doi = {http://dx.doi.org/10.1159/000484524},
keywords = {Catheter; Complex sample; Mixed chromatogram; PCR denaturing gradient gel electrophoresis; RipSeq Mixed},
language = {eng},
issn = {1464-1801},
journal = {Journal of Molecular Microbiology and Biotechnology},
title = {Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis},
volume = {27},
year = {2017}
}
TY - JOUR
ID - 1404252
AU - Kotásková, Iva - Mališová, Barbora - Obručová, Hana - Holá, Veronika - Peroutková, Tereza - Růžička, Filip - Freiberger, Tomáš
PY - 2017
TI - Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
JF - Journal of Molecular Microbiology and Biotechnology
VL - 27
IS - 6
SP - 350-355
EP - 350-355
PB - Karger
SN - 14641801
KW - Catheter
KW - Complex sample
KW - Mixed chromatogram
KW - PCR denaturing gradient gel electrophoresis
KW - RipSeq Mixed
N2 - Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.
ER -
KOTÁSKOVÁ, Iva, Barbora MALIŠOVÁ, Hana OBRUČOVÁ, Veronika HOLÁ, Tereza PEROUTKOVÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis. \textit{Journal of Molecular Microbiology and Biotechnology}. Basel: Karger, roč.~27, č.~6, s.~350-355. ISSN~1464-1801. doi:10.1159/000484524. 2017.
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