Comparative cell cycle transcriptomics reveals synchronization
of developmental transcription factor networks in cancer cells

J 2017

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

BOSTROM, Johan; Zuzana ŠRÁMKOVÁ; Alena SALAŠOVÁ; Helena Anna Dagmar JOHARD; Diana MAHDESSIAN et. al.

Základní údaje

Originální název

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Autoři

Vydání

PLOS ONE, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14740/17:00095459

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1371/journal.pone.0188772

UT WoS

000417648600013

EID Scopus

2-s2.0-85038411630

Klíčová slova anglicky

cell cycle; synchronization; fucci reporter

Štítky

14314010, OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2018 15:55, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.

Návaznosti

EE2.3.20.0180, projekt VaV

Název: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny

GA15-11707S, projekt VaV

Název: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Grantová agentura ČR, Centrosomální abnormality u lidských pluripotentních kmenových buněk

GA15-20818S, projekt VaV

Název: Cílená terapie proti rezistentím kmenovým buňkám gliomového nádoru - kontrola přísunu živin pomocí iontových kanálů

Investor: Grantová agentura ČR, Cílená terapie proti rezistentím kmenovým buňkám gliomového nádoru - kontrola přísunu živin pomocí iontových kanálů

GA15-21789S, projekt VaV

Název: Dishevelled - identifikace základních koordinát

Investor: Grantová agentura ČR, Dishevelled - identifikace základních koordinát

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-33999A, projekt VaV

Název: Vývoj nových nízkomolekulárních protinádorových léčiv na principu syntetické letality

Citovat

BOSTROM, Johan; Zuzana ŠRÁMKOVÁ; Alena SALAŠOVÁ; Helena Anna Dagmar JOHARD; Diana MAHDESSIAN; Radek FEDR; Carolyn MARKS; Jiřina MEDALOVÁ; Karel SOUČEK; Emma LUNDBERG; Sten LINNARSON; Vítězslav BRYJA; Petra SEKYROVÁ; Mikael ALTUN a Michael ANDÄNG. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. PLOS ONE. Public Library of Science, 2017, roč. 12, č. 12, s. 0188772-188795. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0188772.

@article{1405981,
   author = {Bostrom, Johan and Šrámková, Zuzana and Salašová, Alena and Johard, Helena Anna Dagmar and Mahdessian, Diana and Fedr, Radek and Marks, Carolyn and Medalová, Jiřina and Souček, Karel and Lundberg, Emma and Linnarson, Sten and Bryja, Vítězslav and Sekyrová, Petra and Altun, Mikael and Andäng, Michael},
   article_number = {12},
   doi = {https://doi.org/10.1371/journal.pone.0188772},
   keywords = {cell cycle; synchronization; fucci reporter},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1405981
AU  - Bostrom, Johan - Šrámková, Zuzana - Salašová, Alena - Johard, Helena Anna Dagmar - Mahdessian, Diana - Fedr, Radek - Marks, Carolyn - Medalová, Jiřina - Souček, Karel - Lundberg, Emma - Linnarson, Sten - Bryja, Vítězslav - Sekyrová, Petra - Altun, Mikael - Andäng, Michael
PY  - 2017
TI  - Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells
JF  - PLOS ONE
VL  - 12
IS  - 12
SP  - 0188772
EP  - 0188772
PB  - Public Library of Science
SN  - 19326203
KW  - cell cycle
KW  - synchronization
KW  - fucci reporter
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf
N2  - The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.
ER  -

BOSTROM, Johan; Zuzana ŠRÁMKOVÁ; Alena SALAŠOVÁ; Helena Anna Dagmar JOHARD; Diana MAHDESSIAN; Radek FEDR; Carolyn MARKS; Jiřina MEDALOVÁ; Karel SOUČEK; Emma LUNDBERG; Sten LINNARSON; Vítězslav BRYJA; Petra SEKYROVÁ; Mikael ALTUN a Michael ANDÄNG. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. \textit{PLOS ONE}. Public Library of Science, 2017, roč.~12, č.~12, s.~0188772-188795. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0188772.

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