Biological Effects of Drug-Free Alginate Beads Cross-Linked by
Copper Ions Prepared Using External Ionotropic Gelation

J 2017

Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation

PAVELKOVA, M.; K. KUBOVA; Jakub VYSLOUŽIL; M. KEJDUSOVA; D. VETCHY et al.

Základní údaje

Originální název

Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation

Autoři

PAVELKOVA, M. ; K. KUBOVA ; Jakub VYSLOUŽIL ; M. KEJDUSOVA; D. VETCHY; V. CELER; D. MOLINKOVA; D. LOBOVA; A. PECHOVA; Jan VYSLOUŽIL a P. KULICH

Vydání

AAPS PHARMSCITECH, NEW YORK, SPRINGER, 2017, 1530-9932

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.666

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00099800

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

alginate beads; copper's biological effects; dissolution profile; external gelation; vaginal administration

Štítky

NZ, rivok

Změněno: 29. 3. 2018 11:19, Ing. Nicole Zrilić

Anotace

V originále

External ionotropic gelation offers a unique possibility to entrap multivalent ions in a polymer structure. The aim of this experimental study was to prepare new drug-free sodium alginate (ALG) particles cross-linked by Cu2+ ions and to investigate their technological parameters (particle size, sphericity, surface topology, swelling capacity, copper content, release of Cu2+ ions, mucoadhesivity) and biological activity (cytotoxicity and efficiency against the most common vaginal pathogens-Herpes simplex, Escherichia coli, Candida albicans) with respect to potential vaginal administration. Beads prepared fromNaALGdispersions (3 or 4%) were crosslinked by Cu2+ ions (0.5 or 1.0 M CuCl2) using external ionotropic gelation. Prepared mucoadhesive beads with particle size over 1000 mu m exhibited sufficient sphericity (all 0.89) and copper content (214.8-249.07 g/kg), which increased with concentration of polymer and hardening solution. Dissolution behaviour was characterized by extended burst effect, followed by 2 h of copper release. The efficiency of all samples against the most common vaginal pathogens was observed at cytotoxic Cu2+ concentrations. Anti-HSVactivity was demonstrated at a Cu2+ concentration of 546 mg/L. Antibacterial activity of beads (expressed as minimum inhibition concentration, MIC) was influenced mainly by the rate of Cu2+ release which was controlled by the extent of swelling capacity. Lower MIC values were found for E. coli in comparison with C. albicans. Sample ALG-3_1.0 exhibited the fastest copper release and was proved to be the most effective against both bacteria. This could be a result of its lower polymer concentration in combination with smaller particle size and thus larger surface area.

Citovat

PAVELKOVA, M.; K. KUBOVA; Jakub VYSLOUŽIL; M. KEJDUSOVA; D. VETCHY; V. CELER; D. MOLINKOVA; D. LOBOVA; A. PECHOVA; Jan VYSLOUŽIL a P. KULICH. Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation. AAPS PHARMSCITECH. NEW YORK: SPRINGER, 2017, roč. 18, č. 4, s. 1343-1354. ISSN 1530-9932. Dostupné z: https://doi.org/10.1208/s12249-016-0601-4.

@article{1406140,
   author = {Pavelkova, M. and Kubova, K. and Vysloužil, Jakub and Kejdusova, M. and Vetchy, D. and Celer, V. and Molinkova, D. and Lobova, D. and Pechova, A. and Vysloužil, Jan and Kulich, P.},
   article_location = {NEW YORK},
   article_number = {4},
   doi = {https://doi.org/10.1208/s12249-016-0601-4},
   keywords = {alginate beads; copper's biological effects; dissolution profile; external gelation; vaginal administration},
   language = {eng},
   issn = {1530-9932},
   journal = {AAPS PHARMSCITECH},
   title = {Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1406140
AU  - Pavelkova, M. - Kubova, K. - Vysloužil, Jakub - Kejdusova, M. - Vetchy, D. - Celer, V. - Molinkova, D. - Lobova, D. - Pechova, A. - Vysloužil, Jan - Kulich, P.
PY  - 2017
TI  - Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation
JF  - AAPS PHARMSCITECH
VL  - 18
IS  - 4
SP  - 1343-1354
EP  - 1343-1354
PB  - SPRINGER
SN  - 15309932
KW  - alginate beads
KW  - copper's biological effects
KW  - dissolution profile
KW  - external gelation
KW  - vaginal administration
N2  - External ionotropic gelation offers a unique possibility to entrap multivalent ions in a polymer structure. The aim of this experimental study was to prepare new drug-free sodium alginate (ALG) particles cross-linked by Cu2+ ions and to investigate their technological parameters (particle size, sphericity, surface topology, swelling capacity, copper content, release of Cu2+ ions, mucoadhesivity) and biological activity (cytotoxicity and efficiency against the most common vaginal pathogens-Herpes simplex, Escherichia coli, Candida albicans) with respect to potential vaginal administration. Beads prepared fromNaALGdispersions (3 or 4%) were crosslinked by Cu2+ ions (0.5 or 1.0 M CuCl2) using external ionotropic gelation. Prepared mucoadhesive beads with particle size over 1000 mu m exhibited sufficient sphericity (all 0.89) and copper content (214.8-249.07 g/kg), which increased with concentration of polymer and hardening solution. Dissolution behaviour was characterized by extended burst effect, followed by 2 h of copper release. The efficiency of all samples against the most common vaginal pathogens was observed at cytotoxic Cu2+ concentrations. Anti-HSVactivity was demonstrated at a Cu2+ concentration of 546 mg/L. Antibacterial activity of beads (expressed as minimum inhibition concentration, MIC) was influenced mainly by the rate of Cu2+ release which was controlled by the extent of swelling capacity. Lower MIC values were found for E. coli in comparison with C. albicans. Sample ALG-3_1.0 exhibited the fastest copper release and was proved to be the most effective against both bacteria. This could be a result of its lower polymer concentration in combination with smaller particle size and thus larger surface area.
ER  -

PAVELKOVA, M.; K. KUBOVA; Jakub VYSLOUŽIL; M. KEJDUSOVA; D. VETCHY; V. CELER; D. MOLINKOVA; D. LOBOVA; A. PECHOVA; Jan VYSLOUŽIL a P. KULICH. Biological Effects of Drug-Free Alginate Beads Cross-Linked by Copper Ions Prepared Using External Ionotropic Gelation. \textit{AAPS PHARMSCITECH}. NEW YORK: SPRINGER, 2017, roč.~18, č.~4, s.~1343-1354. ISSN~1530-9932. Dostupné z: https://doi.org/10.1208/s12249-016-0601-4.

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