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@article{1407052,
author = {Farka, Zdeněk and Čunderlová, Veronika and Horáčková, Veronika and Pastucha, Matěj and Mikušová, Zuzana and Hlaváček, Antonín and Skládal, Petr},
article_location = {Washington, DC},
article_number = {3},
doi = {http://dx.doi.org/10.1021/acs.analchem.7b04883},
keywords = {Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay},
url = {https://pubs.acs.org/doi/10.1021/acs.analchem.7b04883},
volume = {90},
year = {2018}
}
TY - JOUR
ID - 1407052
AU - Farka, Zdeněk - Čunderlová, Veronika - Horáčková, Veronika - Pastucha, Matěj - Mikušová, Zuzana - Hlaváček, Antonín - Skládal, Petr
PY - 2018
TI - Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay
JF - Analytical Chemistry
VL - 90
IS - 3
SP - 2348-2354
EP - 2348-2354
PB - American Chemical Society
SN - 00032700
KW - Prussian blue nanoparticle
KW - Enzyme biomimic
KW - Bioconjugation
KW - Sandwich immunoassay
KW - Salmonella
KW - Human serum albumin
UR - https://pubs.acs.org/doi/10.1021/acs.analchem.7b04883
N2 - Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3-based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensely blue product. Here, we introduce the method for conjugation of PBNPs with antibodies and their application in nanozyme-linked immunosorbent assay (NLISA). Sandwich NLISA for detection of human serum albumin in urine was developed with limit of detection (LOD) of 1.2 ng·mL–1 and working range up to 1 ug·mL-1. Furthermore, the microbial contamination of Salmonella Typhimurium in powdered milk was detected with LOD of 6 × 10^3 colony-forming units (cfu)·mL-1 and working range up to 10^6 cfu·mL-1. In both cases, a critical comparison with the same immunoassay but using native peroxidase as label was realized. The achieved results confirmed the suitability of PBNPs for universal and robust replacement of enzyme labels.
ER -
FARKA, Zdeněk, Veronika ČUNDERLOVÁ, Veronika HORÁČKOVÁ, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK a Petr SKLÁDAL. Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, roč.~90, č.~3, s.~2348-2354. ISSN~0003-2700. doi:10.1021/acs.analchem.7b04883. 2018.
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