Response of cell lines to actual and simulated inoculation with
Cryptosporidium proliferans

J 2018

Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans

MELICHEROVÁ, Janka; Lada HOFMANNOVÁ a Andrea BARDŮNEK VALIGUROVÁ

Základní údaje

Originální název

Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans

Autoři

MELICHEROVÁ, Janka; Lada HOFMANNOVÁ a Andrea BARDŮNEK VALIGUROVÁ

Vydání

European Journal of Protistology, JENA, ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2018, 0932-4739

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.626

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00100819

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Antigen; Apicomplexa; Cryptosporidium muris; Cryptosporidium proliferans; Encapsulation; Invasion

Klíčová slova anglicky

Antigen; Apicomplexa; Cryptosporidium muris; Cryptosporidium proliferans; Encapsulation; Invasion

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 11:00, Mgr. Michal Petr

Anotace

V originále

The need for an effective treatment against cryptosporidiosis has triggered studies in the search for a working in vitro model. The peculiar niche of cryptosporidia at the brush border of host epithelial cells has been the subject of extensive debates. Despite extensive research on the invasion process, it remains enigmatic whether cryptosporidian host-parasite interactions result from an active invasion process or through encapsulation. We used HCT-8 and HT-29 cell lines for in vitro cultivation of the gastric parasite Cryptosporidium proliferans strain TS03. Using electron and confocal laser scanning microscopy, observations were carried out after 24, 48 and 72 h after inoculation with a mixture of C. proliferans oocysts and sporozoites. Free sporozoites and putative merozoites were observed apparently searching for an appropriate infection site. Advanced stages, corresponding to trophozoites and meronts/gamonts enveloped by parasitophorous sac, and emptied sacs were detected. As our observations showed that even unexcysted oocysts became enveloped by cultured cell projections, using polystyrene microspheres, we evaluated the response of cell lines to simulated inoculation with cryptosporidian oocysts to verify innate and parasite-induced behaviour. We found that cultured cell encapsulation of oocysts is induced by parasite antigens, independent of any active invasion/motility.

Návaznosti

GAP505/11/1163, projekt VaV

Název: Protizánětlivá aktivita extraktů z rostlin Indonésie a jejich účinek na průběh oportunních parazitóz

Investor: Grantová agentura ČR, Protizánětlivá aktivita extraktů z rostlin Indonésie a jejich účinek na průběh oportunních parazitóz

GPP506/10/P372, projekt VaV

Název: Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Investor: Grantová agentura ČR, Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Citovat

MELICHEROVÁ, Janka; Lada HOFMANNOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans. European Journal of Protistology. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2018, roč. 62, FEB, s. 101-121. ISSN 0932-4739. Dostupné z: https://doi.org/10.1016/j.ejop.2017.12.003.

@article{1407706,
   author = {Melicherová, Janka and Hofmannová, Lada and Bardůnek Valigurová, Andrea},
   article_location = {JENA},
   article_number = {FEB},
   doi = {https://doi.org/10.1016/j.ejop.2017.12.003},
   keywords = {Antigen; Apicomplexa; Cryptosporidium muris; Cryptosporidium proliferans; Encapsulation; Invasion},
   language = {eng},
   issn = {0932-4739},
   journal = {European Journal of Protistology},
   title = {Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans},
   url = {https://doi.org/10.1016/j.ejop.2017.12.003},
   volume = {62},
   year = {2018}
}

TY  - JOUR
ID  - 1407706
AU  - Melicherová, Janka - Hofmannová, Lada - Bardůnek Valigurová, Andrea
PY  - 2018
TI  - Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans
JF  - European Journal of Protistology
VL  - 62
IS  - FEB
SP  - 101-121
EP  - 101-121
PB  - ELSEVIER GMBH, URBAN & FISCHER VERLAG
SN  - 09324739
KW  - Antigen
KW  - Apicomplexa
KW  - Cryptosporidium muris
KW  - Cryptosporidium proliferans
KW  - Encapsulation
KW  - Invasion
UR  - https://doi.org/10.1016/j.ejop.2017.12.003
N2  - The need for an effective treatment against cryptosporidiosis has triggered studies in the search for a working in vitro model. The peculiar niche of cryptosporidia at the brush border of host epithelial cells has been the subject of extensive debates. Despite extensive research on the invasion process, it remains enigmatic whether cryptosporidian host-parasite interactions result from an active invasion process or through encapsulation. We used HCT-8 and HT-29 cell lines for in vitro cultivation of the gastric parasite Cryptosporidium proliferans strain TS03. Using electron and confocal laser scanning microscopy, observations were carried out after 24, 48 and 72 h after inoculation with a mixture of C. proliferans oocysts and sporozoites. Free sporozoites and putative merozoites were observed apparently searching for an appropriate infection site. Advanced stages, corresponding to trophozoites and meronts/gamonts enveloped by parasitophorous sac, and emptied sacs were detected. As our observations showed that even unexcysted oocysts became enveloped by cultured cell projections, using polystyrene microspheres, we evaluated the response of cell lines to simulated inoculation with cryptosporidian oocysts to verify innate and parasite-induced behaviour. We found that cultured cell encapsulation of oocysts is induced by parasite antigens, independent of any active invasion/motility.
ER  -

MELICHEROVÁ, Janka; Lada HOFMANNOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans. \textit{European Journal of Protistology}. JENA: ELSEVIER GMBH, URBAN \&{}amp; FISCHER VERLAG, 2018, roč.~62, FEB, s.~101-121. ISSN~0932-4739. Dostupné z: https://doi.org/10.1016/j.ejop.2017.12.003.

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