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@article{1407745,
author = {Komendová, Martina and Metelka, Radovan and Urban, Jiří},
article_location = {Amsterdam, Netherlands},
article_number = {AUG 2017},
doi = {http://dx.doi.org/10.1016/j.chroma.2017.06.057},
keywords = {Dopamine; Electrochemical detection; Integrated column; Polymer monoliths; Urine},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Monolithic capillary column with an integrated electrochemical detector},
url = {https://www.sciencedirect.com/science/article/pii/S0021967317309251?via%3Dihub},
volume = {1509},
year = {2017}
}
TY - JOUR
ID - 1407745
AU - Komendová, Martina - Metelka, Radovan - Urban, Jiří
PY - 2017
TI - Monolithic capillary column with an integrated electrochemical detector
JF - Journal of Chromatography A
VL - 1509
IS - AUG 2017
SP - 171-175
EP - 171-175
PB - Elsevier
SN - 00219673
KW - Dopamine
KW - Electrochemical detection
KW - Integrated column
KW - Polymer monoliths
KW - Urine
UR - https://www.sciencedirect.com/science/article/pii/S0021967317309251?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0021967317309251?via%3Dihub
N2 - The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0 mg L-1 and an instrumental limit of detection as low as 24 pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74 +/- 0.03 mgL(-1) and 0.71 +/- 0.02 mgL(-1), respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days. (C) 2017 Elsevier B.V. All rights reserved.
ER -
KOMENDOVÁ, Martina, Radovan METELKA a Jiří URBAN. Monolithic capillary column with an integrated electrochemical detector. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2017, roč.~1509, AUG 2017, s.~171-175. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2017.06.057.
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