Antibody-mediated modulation of cytokinins in tobacco:
organ-specific changes in cytokinin homeostasis

J 2018

Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis

GELOVÁ, Zuzana; Petra TEN HOOPEN; Ondřej NOVÁK; Václav MOTYKA; Markéta PERNISOVÁ et al.

Základní údaje

Originální název

Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis

Autoři

Vydání

Journal of Experimental Botany, Clarendon Press, 2018, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.360

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00100822

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

antibody-mediated modulation; biosynthesis; CKX; cytokinin; homeostasis; organ specificity; tobacco.

Štítky

CF MAFIL, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 12:49, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation.

Návaznosti

GA13-25280S, projekt VaV

Název: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Grantová agentura ČR, Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

GP14-30004P, projekt VaV

Název: Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.

Investor: Grantová agentura ČR, Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.

LM2015062, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

GELOVÁ, Zuzana; Petra TEN HOOPEN; Ondřej NOVÁK; Václav MOTYKA; Markéta PERNISOVÁ; Siarhei DABRAVOLSKI; Vojtěch DIDI; Isolde TILLACK; Jana OKLEŠŤKOVÁ; Miroslav STRNAD; Bettina HAUSE; Danka HARUŠTIAKOVÁ; Udo CONRAD; Lubomír JANDA a Jan HEJÁTKO. Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. Journal of Experimental Botany. Clarendon Press, 2018, roč. 69, č. 3, s. 441-454. ISSN 0022-0957. Dostupné z: https://doi.org/10.1093/jxb/erx426.

@article{1407869,
   author = {Gelová, Zuzana and ten Hoopen, Petra and Novák, Ondřej and Motyka, Václav and Pernisová, Markéta and Dabravolski, Siarhei and Didi, Vojtěch and Tillack, Isolde and Oklešťková, Jana and Strnad, Miroslav and Hause, Bettina and Haruštiaková, Danka and Conrad, Udo and Janda, Lubomír and Hejátko, Jan},
   article_number = {3},
   doi = {https://doi.org/10.1093/jxb/erx426},
   keywords = {antibody-mediated modulation; biosynthesis; CKX; cytokinin; homeostasis; organ specificity; tobacco.},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis},
   volume = {69},
   year = {2018}
}

TY  - JOUR
ID  - 1407869
AU  - Gelová, Zuzana - ten Hoopen, Petra - Novák, Ondřej - Motyka, Václav - Pernisová, Markéta - Dabravolski, Siarhei - Didi, Vojtěch - Tillack, Isolde - Oklešťková, Jana - Strnad, Miroslav - Hause, Bettina - Haruštiaková, Danka - Conrad, Udo - Janda, Lubomír - Hejátko, Jan
PY  - 2018
TI  - Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis
JF  - Journal of Experimental Botany
VL  - 69
IS  - 3
SP  - 441-454
EP  - 441-454
PB  - Clarendon Press
SN  - 00220957
KW  - antibody-mediated modulation
KW  - biosynthesis
KW  - CKX
KW  - cytokinin
KW  - homeostasis
KW  - organ specificity
KW  - tobacco.
N2  - Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation.
ER  -

GELOVÁ, Zuzana; Petra TEN HOOPEN; Ondřej NOVÁK; Václav MOTYKA; Markéta PERNISOVÁ; Siarhei DABRAVOLSKI; Vojtěch DIDI; Isolde TILLACK; Jana OKLEŠŤKOVÁ; Miroslav STRNAD; Bettina HAUSE; Danka HARUŠTIAKOVÁ; Udo CONRAD; Lubomír JANDA a Jan HEJÁTKO. Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. \textit{Journal of Experimental Botany}. Clarendon Press, 2018, roč.~69, č.~3, s.~441-454. ISSN~0022-0957. Dostupné z: https://doi.org/10.1093/jxb/erx426.

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