Retention of small molecules on polymethacrylate monolithic
capillary columns

J 2017

Retention of small molecules on polymethacrylate monolithic capillary columns

CHOCHOLOUŠKOVÁ, Michaela; Martina KOMENDOVÁ a Jiří URBAN

Základní údaje

Originální název

Retention of small molecules on polymethacrylate monolithic capillary columns

Autoři

CHOCHOLOUŠKOVÁ, Michaela; Martina KOMENDOVÁ a Jiří URBAN

Vydání

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2017, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.716

Označené pro přenos do RIV

DOI

https://doi.org/10.1016/j.chroma.2017.01.079

UT WoS

000395853700010

Klíčová slova anglicky

N-isopropylacrylamide; Polymer monoliths; Retention; Small molecules; Temperature

Změněno: 16. 2. 2018 14:02, prof. RNDr. Jiří Urban, Ph.D.

Anotace

V originále

In this paper, the concentration of N-isopropylacrylamide in the polymerization mixture has been varied to prepare several polymethacrylate monolithic capillary columns. Polymer monoliths combining N-isopropylacrylamide with zwitterion monomer, as well as various dimethacrylate crosslinking monomers have been prepared and characterized. Uracil, thiourea, phenol, toluene, ethylbenzene, propylbenzene, and butylbenzene have been used to characterize retention of prepared capillary columns in the mobile phases with 40-95% of acetonitrile and at working temperatures ranging from 25 to 60 degrees C. By an optimization of six-parameter polynomial models we have found that the retention of small molecules is affected mainly by the concentration of the acetonitrile in the mobile phase with very low contribution of working temperature and combined effect of acetonitrile concentration and temperature. Concentration of the mobile phase controlled also enthalpy of the retention. On the other hand, entropic contribution was almost insensitive to the change of the mobile phase composition, especially for mobile phases containing more than 60% of acetonitrile. (C) 2017 Elsevier B.V. All rights reserved.

Citovat

CHOCHOLOUŠKOVÁ, Michaela; Martina KOMENDOVÁ a Jiří URBAN. Retention of small molecules on polymethacrylate monolithic capillary columns. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2017, roč. 1488, s. 85-92. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2017.01.079.

@article{1407959,
   author = {Chocholoušková, Michaela and Komendová, Martina and Urban, Jiří},
   article_location = {Amsterdam, Netherlands},
   doi = {https://doi.org/10.1016/j.chroma.2017.01.079},
   keywords = {N-isopropylacrylamide; Polymer monoliths; Retention; Small molecules; Temperature},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Retention of small molecules on polymethacrylate monolithic capillary columns},
   url = {http://dx.doi.org/10.1016/j.chroma.2017.01.079},
   volume = {1488},
   year = {2017}
}

TY  - JOUR
ID  - 1407959
AU  - Chocholoušková, Michaela - Komendová, Martina - Urban, Jiří
PY  - 2017
TI  - Retention of small molecules on polymethacrylate monolithic capillary columns
JF  - Journal of Chromatography A
VL  - 1488
SP  - 85-92
EP  - 85-92
PB  - Elsevier
SN  - 00219673
KW  - N-isopropylacrylamide
KW  - Polymer monoliths
KW  - Retention
KW  - Small molecules
KW  - Temperature
UR  - http://dx.doi.org/10.1016/j.chroma.2017.01.079
L2  - http://dx.doi.org/10.1016/j.chroma.2017.01.079
N2  - In this paper, the concentration of N-isopropylacrylamide in the polymerization mixture has been varied to prepare several polymethacrylate monolithic capillary columns. Polymer monoliths combining N-isopropylacrylamide with zwitterion monomer, as well as various dimethacrylate crosslinking monomers have been prepared and characterized. Uracil, thiourea, phenol, toluene, ethylbenzene, propylbenzene, and butylbenzene have been used to characterize retention of prepared capillary columns in the mobile phases with 40-95% of acetonitrile and at working temperatures ranging from 25 to 60 degrees C. By an optimization of six-parameter polynomial models we have found that the retention of small molecules is affected mainly by the concentration of the acetonitrile in the mobile phase with very low contribution of working temperature and combined effect of acetonitrile concentration and temperature. Concentration of the mobile phase controlled also enthalpy of the retention. On the other hand, entropic contribution was almost insensitive to the change of the mobile phase composition, especially for mobile phases containing more than 60% of acetonitrile. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

CHOCHOLOUŠKOVÁ, Michaela; Martina KOMENDOVÁ a Jiří URBAN. Retention of small molecules on polymethacrylate monolithic capillary columns. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2017, roč.~1488, s.~85-92. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2017.01.079.

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