Development of an online solid-phase extraction with liquid
chromatography method based on polymer monoliths for the
determination of dopamine

J 2016

Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine

JANKŮ, Simona; Martina KOMENDOVÁ a Jiří URBAN

Základní údaje

Originální název

Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine

Autoři

JANKŮ, Simona; Martina KOMENDOVÁ a Jiří URBAN

Vydání

Journal of Separation Science, Weinham, WILEY-VCH Verlag GmbH & Co. 2016, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.557

Označené pro přenos do RIV

DOI

https://doi.org/10.1002/jssc.201600818

UT WoS

000389046300006

Klíčová slova anglicky

Design of experiments; Dopamine; Monomers; Polymer monoliths; Solid-phase extraction

Štítky

RIV ne

Změněno: 16. 2. 2018 14:04, prof. RNDr. Jiří Urban, Ph.D.

Anotace

V originále

Porous polymer monoliths have been used to develop an online solid-phase extraction with liquid chromatography method for determination of dopamine in urine as well as for a continuous monitoring of dopamine in flowing system. A polymerization mixture containing 4-vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine cis-diol functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow-rate and a flow-rate of flushing buffer were selected as the most significant variables. Optimized experimental setup was applied to continuously monitor dopamine degradation.

Citovat

JANKŮ, Simona; Martina KOMENDOVÁ a Jiří URBAN. Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine. Journal of Separation Science. Weinham: WILEY-VCH Verlag GmbH & Co., 2016, roč. 39, č. 21, s. 4107-4115. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201600818.

@article{1407960,
   author = {Janků, Simona and Komendová, Martina and Urban, Jiří},
   article_location = {Weinham},
   article_number = {21},
   doi = {https://doi.org/10.1002/jssc.201600818},
   keywords = {Design of experiments; Dopamine; Monomers; Polymer monoliths; Solid-phase extraction},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine},
   url = {http://dx.doi.org/10.1002/jssc.201600818},
   volume = {39},
   year = {2016}
}

TY  - JOUR
ID  - 1407960
AU  - Janků, Simona - Komendová, Martina - Urban, Jiří
PY  - 2016
TI  - Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
JF  - Journal of Separation Science
VL  - 39
IS  - 21
SP  - 4107-4115
EP  - 4107-4115
PB  - WILEY-VCH Verlag GmbH & Co.
SN  - 16159306
KW  - Design of experiments
KW  - Dopamine
KW  - Monomers
KW  - Polymer monoliths
KW  - Solid-phase extraction
UR  - http://dx.doi.org/10.1002/jssc.201600818
L2  - http://dx.doi.org/10.1002/jssc.201600818
N2  - Porous polymer monoliths have been used to develop an online solid-phase extraction with liquid chromatography method for determination of dopamine in urine as well as for a continuous monitoring of dopamine in flowing system. A polymerization mixture containing 4-vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine cis-diol functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow-rate and a flow-rate of flushing buffer were selected as the most significant variables. Optimized experimental setup was applied to continuously monitor dopamine degradation.
ER  -

JANKŮ, Simona; Martina KOMENDOVÁ a Jiří URBAN. Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine. \textit{Journal of Separation Science}. Weinham: WILEY-VCH Verlag GmbH \&{} Co., 2016, roč.~39, č.~21, s.~4107-4115. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201600818.

Přehled o publikaci