A novel liquid chromatography/mass spectrometry method for
determination of neurotransmitters in brain tissue: Application
to human tauopathies

J 2018

A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies

FORGACSOVA, Andrea; Jaroslav GALBA; Ralph M. GARRUTO; Petra MAJEROVA; Stanislav KATINA et al.

Základní údaje

Originální název

A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies

Autoři

FORGACSOVA, Andrea; Jaroslav GALBA; Ralph M. GARRUTO; Petra MAJEROVA; Stanislav KATINA a Andrej KOVAC

Vydání

Journal of Chromatography B, United States, Elsevier, 2018, 1570-0232

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10103 Statistics and probability

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.813

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00102323

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Neurotransmitters; UHPLC/MS; Human brain tissue; Tauopathies

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2020 13:24, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Neurotransmitters, small molecules widely distributed in the central nervous system are essential in transmitting electrical signals across neurons via chemical communication. Dysregulation of these chemical signaling molecules is linked to numerous neurological diseases including tauopathies. In this study, a precise and reliable liquid chromatography method was established with tandem mass spectrometry detection for the simultaneous determination of aspartic acid, asparagine, glutamic acid, glutamine, gamma-aminobutyric acid, N-acetyl-l-aspartic acid, pyroglutamic acid, acetylcholine and choline in human brain tissue. The method was successfully applied to the analysis of human brain tissues from three different tauopathies; corticobasal degeneration, progressive supranuclear palsy and parkinsonism-dementia complex of Guam. Neurotransmitters were analyzed on ultra-high performance chromatography (UHPLC) using an ethylene bridged hybrid amide column coupled with tandem mass spectrometry (MS/MS). Identification and quantification of neurotransmitters was carried out by ESI+ mass spectrometry detection. We optimized sample preparation to achieve simple and fast extraction of all nine analytes. Our method exhibited an excellent linearity for all analytes (all coefficients of determination more than 0.99), with inter-day and intra-day precision yielding relative standard deviations 3.2%–11.2% and an accuracy was in range of 92.6%–104.3%. The present study, using the above method, is the first to demonstrate significant alterations of brain neurotransmitters caused by pathological processes in the brain tissues of patient with three different tauopathies.

Citovat

FORGACSOVA, Andrea; Jaroslav GALBA; Ralph M. GARRUTO; Petra MAJEROVA; Stanislav KATINA a Andrej KOVAC. A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies. Journal of Chromatography B. United States: Elsevier, 2018, roč. 1073, č. 1, s. 154-162. ISSN 1570-0232. Dostupné z: https://doi.org/10.1016/j.jchromb.2017.12.015.

@article{1408591,
   author = {Forgacsova, Andrea and Galba, Jaroslav and Garruto, Ralph M. and Majerova, Petra and Katina, Stanislav and Kovac, Andrej},
   article_location = {United States},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.jchromb.2017.12.015},
   keywords = {Neurotransmitters; UHPLC/MS; Human brain tissue; Tauopathies},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B},
   title = {A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies},
   url = {https://www.sciencedirect.com/science/article/pii/S1570023217315362?via%3Dihub},
   volume = {1073},
   year = {2018}
}

TY  - JOUR
ID  - 1408591
AU  - Forgacsova, Andrea - Galba, Jaroslav - Garruto, Ralph M. - Majerova, Petra - Katina, Stanislav - Kovac, Andrej
PY  - 2018
TI  - A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies
JF  - Journal of Chromatography B
VL  - 1073
IS  - 1
SP  - 154-162
EP  - 154-162
PB  - Elsevier
SN  - 15700232
KW  - Neurotransmitters
KW  - UHPLC/MS
KW  - Human brain tissue
KW  - Tauopathies
UR  - https://www.sciencedirect.com/science/article/pii/S1570023217315362?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S1570023217315362?via%3Dihub
N2  - Neurotransmitters, small molecules widely distributed in the central nervous system are essential in transmitting electrical signals across neurons via chemical communication. Dysregulation of these chemical signaling molecules is linked to numerous neurological diseases including tauopathies. In this study, a precise and reliable liquid chromatography method was established with tandem mass spectrometry detection for the simultaneous determination of aspartic acid, asparagine, glutamic acid, glutamine, gamma-aminobutyric acid, N-acetyl-l-aspartic acid, pyroglutamic acid, acetylcholine and choline in human brain tissue. The method was successfully applied to the analysis of human brain tissues from three different tauopathies; corticobasal degeneration, progressive supranuclear palsy and parkinsonism-dementia complex of Guam. Neurotransmitters were analyzed on ultra-high performance chromatography (UHPLC) using an ethylene bridged hybrid amide column coupled with tandem mass spectrometry (MS/MS). Identification and quantification of neurotransmitters was carried out by ESI+ mass spectrometry detection. We optimized sample preparation to achieve simple and fast extraction of all nine analytes. Our method exhibited an excellent linearity for all analytes (all coefficients of determination more than 0.99), with inter-day and intra-day precision yielding relative standard deviations 3.2%–11.2% and an accuracy was in range of 92.6%–104.3%. The present study, using the above method, is the first to demonstrate significant alterations of brain neurotransmitters caused by pathological processes in the brain tissues of patient with three different tauopathies.
ER  -

FORGACSOVA, Andrea; Jaroslav GALBA; Ralph M. GARRUTO; Petra MAJEROVA; Stanislav KATINA a Andrej KOVAC. A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies. \textit{Journal of Chromatography B}. United States: Elsevier, 2018, roč.~1073, č.~1, s.~154-162. ISSN~1570-0232. Dostupné z: https://doi.org/10.1016/j.jchromb.2017.12.015.

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