FORGACSOVA, Andrea, Jaroslav GALBA, Ralph M. GARRUTO, Petra MAJEROVA, Stanislav KATINA a Andrej KOVAC. A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies. Journal of Chromatography B. United States: Elsevier, 2018, roč. 1073, č. 1, s. 154-162. ISSN 1570-0232. Dostupné z: https://dx.doi.org/10.1016/j.jchromb.2017.12.015.
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Základní údaje
Originální název A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies
Autoři FORGACSOVA, Andrea (703 Slovensko), Jaroslav GALBA (703 Slovensko), Ralph M. GARRUTO (840 Spojené státy), Petra MAJEROVA (703 Slovensko), Stanislav KATINA (703 Slovensko, garant, domácí) a Andrej KOVAC (703 Slovensko).
Vydání Journal of Chromatography B, United States, Elsevier, 2018, 1570-0232.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10103 Statistics and probability
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 2.813
Kód RIV RIV/00216224:14310/18:00102323
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.1016/j.jchromb.2017.12.015
UT WoS 000423889300020
Klíčová slova anglicky Neurotransmitters; UHPLC/MS; Human brain tissue; Tauopathies
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 8. 4. 2020 13:24.
Anotace
Neurotransmitters, small molecules widely distributed in the central nervous system are essential in transmitting electrical signals across neurons via chemical communication. Dysregulation of these chemical signaling molecules is linked to numerous neurological diseases including tauopathies. In this study, a precise and reliable liquid chromatography method was established with tandem mass spectrometry detection for the simultaneous determination of aspartic acid, asparagine, glutamic acid, glutamine, gamma-aminobutyric acid, N-acetyl-l-aspartic acid, pyroglutamic acid, acetylcholine and choline in human brain tissue. The method was successfully applied to the analysis of human brain tissues from three different tauopathies; corticobasal degeneration, progressive supranuclear palsy and parkinsonism-dementia complex of Guam. Neurotransmitters were analyzed on ultra-high performance chromatography (UHPLC) using an ethylene bridged hybrid amide column coupled with tandem mass spectrometry (MS/MS). Identification and quantification of neurotransmitters was carried out by ESI+ mass spectrometry detection. We optimized sample preparation to achieve simple and fast extraction of all nine analytes. Our method exhibited an excellent linearity for all analytes (all coefficients of determination more than 0.99), with inter-day and intra-day precision yielding relative standard deviations 3.2%–11.2% and an accuracy was in range of 92.6%–104.3%. The present study, using the above method, is the first to demonstrate significant alterations of brain neurotransmitters caused by pathological processes in the brain tissues of patient with three different tauopathies.
VytisknoutZobrazeno: 19. 9. 2024 15:43