Tissue-specific expression of telomerase reverse transcriptase
gene variants in Nicotiana tabacum

J 2017

Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS et. al.

Basic information

Original name

Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum

Authors

JUREČKOVÁ, Jana (203 Czech Republic, belonging to the institution); E. SYKOROVA (203 Czech Republic); S. HAFIDH (784 United Arab Emirates); D. HONYS (203 Czech Republic); Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution)

Edition

Planta, NEW YORK, SPRINGER, 2017, 0032-0935

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

Full Text

Impact factor

Impact factor: 3.249

RIV identification code

RIV/00216224:14740/17:00095505

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00425-016-2624-1

UT WoS

000394436100006

EID Scopus

2-s2.0-85000786866

Keywords in English

Gene sequence variant; Pollen; Polyploids; Telomerase; Telomere; Transcription

Abstract

In the original language

In tobacco, three sequence variants of the TERT gene have been described. We revealed unbalanced levels of TERT variant transcripts in vegetative tobacco tissues and enhanced TERT transcription and telomerase activity in reproductive tissues. Telomerase is a ribonucleoprotein complex responsible for the maintenance of telomeres, structures delimiting ends of linear eukaryotic chromosomes. In the Nicotiana tabacum (tobacco) allotetraploid plant, three sequence variants (paralogs) of the gene coding for the telomerase reverse transcriptase subunit (TERT) have been described, two of them derived from the maternal N. sylvestris genome (TERT_Cs, TERT_D) and one originated from the N. tomentosiformis paternal genome (TERT_Ct). In this work, we analyzed the transcription of TERT variants in correlation with telomerase activity in tobacco tissues. High and approximately comparable levels of TERT_Ct and TERT_Cs transcripts were detected in seedlings, roots, flower buds and leaves, while the transcript of the TERT_D variant was markedly underrepresented. Similarly, in N. sylvestris tissues, TERT_Cs transcript significantly predominated. A specific pattern of TERT transcripts was found in samples of tobacco pollen with the TERT_Cs variant clearly dominating particularly at the early stage of pollen development. Detailed analysis of TERT_C variants representation in functionally distinct fractions of pollen transcriptome revealed their prevalence in large ribonucleoprotein particles encompassing translationally silent mRNA; only a minority of TERT_Ct and TERT_Cs transcripts were localized in actively translated polysomes. Histones of the TERT_C chromatin were decorated predominantly with the euchromatin-specific epigenetic modification in both telomerase-positive and telomerase-negative tobacco tissues. We conclude that the existence and transcription pattern of tobacco TERT paralogs represents an interesting phenomenon and our results indicate its functional significance. Nicotiana species have again proved to be appropriate and useful model plants in telomere biology studies.

Links

GA13-06943S, research and development project

Name: Strukturní a funkční komponenty rostlinných telomer

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Cite

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS and Miloslava FOJTOVÁ. Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum. Planta. NEW YORK: SPRINGER, 2017, vol. 245, No 3, p. 549-561. ISSN 0032-0935. Available from: https://dx.doi.org/10.1007/s00425-016-2624-1.

@article{1408637,
   author = {Jurečková, Jana and Sykorova, E. and Hafidh, S. and Honys, D. and Fajkus, Jiří and Fojtová, Miloslava},
   article_location = {NEW YORK},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00425-016-2624-1},
   keywords = {Gene sequence variant; Pollen; Polyploids; Telomerase; Telomere; Transcription},
   language = {eng},
   issn = {0032-0935},
   journal = {Planta},
   title = {Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum},
   url = {https://link.springer.com/article/10.1007/s00425-016-2624-1},
   volume = {245},
   year = {2017}
}

TY  - JOUR
ID  - 1408637
AU  - Jurečková, Jana - Sykorova, E. - Hafidh, S. - Honys, D. - Fajkus, Jiří - Fojtová, Miloslava
PY  - 2017
TI  - Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum
JF  - Planta
VL  - 245
IS  - 3
SP  - 549-561
EP  - 549-561
PB  - SPRINGER
SN  - 00320935
KW  - Gene sequence variant
KW  - Pollen
KW  - Polyploids
KW  - Telomerase
KW  - Telomere
KW  - Transcription
UR  - https://link.springer.com/article/10.1007/s00425-016-2624-1
L2  - https://link.springer.com/article/10.1007/s00425-016-2624-1
N2  - In tobacco, three sequence variants of the TERT gene have been described. We revealed unbalanced levels of TERT variant transcripts in vegetative tobacco tissues and enhanced TERT transcription and telomerase activity in reproductive tissues. Telomerase is a ribonucleoprotein complex responsible for the maintenance of telomeres, structures delimiting ends of linear eukaryotic chromosomes. In the Nicotiana tabacum (tobacco) allotetraploid plant, three sequence variants (paralogs) of the gene coding for the telomerase reverse transcriptase subunit (TERT) have been described, two of them derived from the maternal N. sylvestris genome (TERT_Cs, TERT_D) and one originated from the N. tomentosiformis paternal genome (TERT_Ct). In this work, we analyzed the transcription of TERT variants in correlation with telomerase activity in tobacco tissues. High and approximately comparable levels of TERT_Ct and TERT_Cs transcripts were detected in seedlings, roots, flower buds and leaves, while the transcript of the TERT_D variant was markedly underrepresented. Similarly, in N. sylvestris tissues, TERT_Cs transcript significantly predominated. A specific pattern of TERT transcripts was found in samples of tobacco pollen with the TERT_Cs variant clearly dominating particularly at the early stage of pollen development. Detailed analysis of TERT_C variants representation in functionally distinct fractions of pollen transcriptome revealed their prevalence in large ribonucleoprotein particles encompassing translationally silent mRNA; only a minority of TERT_Ct and TERT_Cs transcripts were localized in actively translated polysomes. Histones of the TERT_C chromatin were decorated predominantly with the euchromatin-specific epigenetic modification in both telomerase-positive and telomerase-negative tobacco tissues. We conclude that the existence and transcription pattern of tobacco TERT paralogs represents an interesting phenomenon and our results indicate its functional significance. Nicotiana species have again proved to be appropriate and useful model plants in telomere biology studies.
ER  -

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS and Miloslava FOJTOVÁ. Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum. \textit{Planta}. NEW YORK: SPRINGER, 2017, vol.~245, No~3, p.~549-561. ISSN~0032-0935. Available from: https://dx.doi.org/10.1007/s00425-016-2624-1.

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