Tissue-specific expression of telomerase reverse transcriptase
gene variants in Nicotiana tabacum

J 2017

Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS et. al.

Základní údaje

Originální název

Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum

Autoři

JUREČKOVÁ, Jana (203 Česká republika, domácí); E. SYKOROVA (203 Česká republika); S. HAFIDH (784 Spojené arabské emiráty); D. HONYS (203 Česká republika); Jiří FAJKUS (203 Česká republika, garant, domácí) a Miloslava FOJTOVÁ (203 Česká republika, domácí)

Vydání

Planta, NEW YORK, SPRINGER, 2017, 0032-0935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.249

Kód RIV

RIV/00216224:14740/17:00095505

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00425-016-2624-1

UT WoS

000394436100006

EID Scopus

2-s2.0-85000786866

Klíčová slova anglicky

Gene sequence variant; Pollen; Polyploids; Telomerase; Telomere; Transcription

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2019 17:47, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In tobacco, three sequence variants of the TERT gene have been described. We revealed unbalanced levels of TERT variant transcripts in vegetative tobacco tissues and enhanced TERT transcription and telomerase activity in reproductive tissues. Telomerase is a ribonucleoprotein complex responsible for the maintenance of telomeres, structures delimiting ends of linear eukaryotic chromosomes. In the Nicotiana tabacum (tobacco) allotetraploid plant, three sequence variants (paralogs) of the gene coding for the telomerase reverse transcriptase subunit (TERT) have been described, two of them derived from the maternal N. sylvestris genome (TERT_Cs, TERT_D) and one originated from the N. tomentosiformis paternal genome (TERT_Ct). In this work, we analyzed the transcription of TERT variants in correlation with telomerase activity in tobacco tissues. High and approximately comparable levels of TERT_Ct and TERT_Cs transcripts were detected in seedlings, roots, flower buds and leaves, while the transcript of the TERT_D variant was markedly underrepresented. Similarly, in N. sylvestris tissues, TERT_Cs transcript significantly predominated. A specific pattern of TERT transcripts was found in samples of tobacco pollen with the TERT_Cs variant clearly dominating particularly at the early stage of pollen development. Detailed analysis of TERT_C variants representation in functionally distinct fractions of pollen transcriptome revealed their prevalence in large ribonucleoprotein particles encompassing translationally silent mRNA; only a minority of TERT_Ct and TERT_Cs transcripts were localized in actively translated polysomes. Histones of the TERT_C chromatin were decorated predominantly with the euchromatin-specific epigenetic modification in both telomerase-positive and telomerase-negative tobacco tissues. We conclude that the existence and transcription pattern of tobacco TERT paralogs represents an interesting phenomenon and our results indicate its functional significance. Nicotiana species have again proved to be appropriate and useful model plants in telomere biology studies.

Návaznosti

GA13-06943S, projekt VaV

Název: Strukturní a funkční komponenty rostlinných telomer

Investor: Grantová agentura ČR, Strukturní a funkční komponenty rostlinných telomer

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS a Miloslava FOJTOVÁ. Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum. Planta. NEW YORK: SPRINGER, 2017, roč. 245, č. 3, s. 549-561. ISSN 0032-0935. Dostupné z: https://dx.doi.org/10.1007/s00425-016-2624-1.

@article{1408637,
   author = {Jurečková, Jana and Sykorova, E. and Hafidh, S. and Honys, D. and Fajkus, Jiří and Fojtová, Miloslava},
   article_location = {NEW YORK},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00425-016-2624-1},
   keywords = {Gene sequence variant; Pollen; Polyploids; Telomerase; Telomere; Transcription},
   language = {eng},
   issn = {0032-0935},
   journal = {Planta},
   title = {Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum},
   url = {https://link.springer.com/article/10.1007/s00425-016-2624-1},
   volume = {245},
   year = {2017}
}

TY  - JOUR
ID  - 1408637
AU  - Jurečková, Jana - Sykorova, E. - Hafidh, S. - Honys, D. - Fajkus, Jiří - Fojtová, Miloslava
PY  - 2017
TI  - Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum
JF  - Planta
VL  - 245
IS  - 3
SP  - 549-561
EP  - 549-561
PB  - SPRINGER
SN  - 00320935
KW  - Gene sequence variant
KW  - Pollen
KW  - Polyploids
KW  - Telomerase
KW  - Telomere
KW  - Transcription
UR  - https://link.springer.com/article/10.1007/s00425-016-2624-1
L2  - https://link.springer.com/article/10.1007/s00425-016-2624-1
N2  - In tobacco, three sequence variants of the TERT gene have been described. We revealed unbalanced levels of TERT variant transcripts in vegetative tobacco tissues and enhanced TERT transcription and telomerase activity in reproductive tissues. Telomerase is a ribonucleoprotein complex responsible for the maintenance of telomeres, structures delimiting ends of linear eukaryotic chromosomes. In the Nicotiana tabacum (tobacco) allotetraploid plant, three sequence variants (paralogs) of the gene coding for the telomerase reverse transcriptase subunit (TERT) have been described, two of them derived from the maternal N. sylvestris genome (TERT_Cs, TERT_D) and one originated from the N. tomentosiformis paternal genome (TERT_Ct). In this work, we analyzed the transcription of TERT variants in correlation with telomerase activity in tobacco tissues. High and approximately comparable levels of TERT_Ct and TERT_Cs transcripts were detected in seedlings, roots, flower buds and leaves, while the transcript of the TERT_D variant was markedly underrepresented. Similarly, in N. sylvestris tissues, TERT_Cs transcript significantly predominated. A specific pattern of TERT transcripts was found in samples of tobacco pollen with the TERT_Cs variant clearly dominating particularly at the early stage of pollen development. Detailed analysis of TERT_C variants representation in functionally distinct fractions of pollen transcriptome revealed their prevalence in large ribonucleoprotein particles encompassing translationally silent mRNA; only a minority of TERT_Ct and TERT_Cs transcripts were localized in actively translated polysomes. Histones of the TERT_C chromatin were decorated predominantly with the euchromatin-specific epigenetic modification in both telomerase-positive and telomerase-negative tobacco tissues. We conclude that the existence and transcription pattern of tobacco TERT paralogs represents an interesting phenomenon and our results indicate its functional significance. Nicotiana species have again proved to be appropriate and useful model plants in telomere biology studies.
ER  -

JUREČKOVÁ, Jana; E. SYKOROVA; S. HAFIDH; D. HONYS; Jiří FAJKUS a Miloslava FOJTOVÁ. Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum. \textit{Planta}. NEW YORK: SPRINGER, 2017, roč.~245, č.~3, s.~549-561. ISSN~0032-0935. Dostupné z: https://dx.doi.org/10.1007/s00425-016-2624-1.

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