Cytokines, growth, and environment factors in bone marrow
plasma of acute lymphoblastic leukemia pediatric patients

J 2014

Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients

KOVAC, M; M VASKOVA; D PETRACKOVA; Vendula PELKOVÁ; E MEJSTRIKOVA et al.

Základní údaje

Originální název

Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients

Autoři

KOVAC, M; M VASKOVA; D PETRACKOVA; Vendula PELKOVÁ; E MEJSTRIKOVA; T KALINA; M ZALIOVA; J WEISER; J STARY a O HRUSAK

Vydání

EUROPEAN CYTOKINE NETWORK, MONTROUGE, JOHN LIBBEY EUROTEXT LTD, 2014, 1148-5493

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.960

Označené pro přenos do RIV

DOI

https://doi.org/10.1684/ecn.2014.0348

UT WoS

000341329400002

Klíčová slova anglicky

pediatric acute lymphoblastic leukemia; bone marrow plasma; cytokine antibody array

Změněno: 23. 2. 2018 10:03, Mgr. Vendula Pelková

Anotace

V originále

Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of ALL cells. LBMp (leukemic bone marrow plasma) at the time of ALL diagnosis was compared to control plasma of bone marrow (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The cytokine antibody array enabled simultaneous detection of 79 proteins per sample. Candidate proteins exhibiting significantly different profiles were further analyzed and confirmed by ELISA. mRNAexpression of one of the candidate proteins (TIMP1) was studied using quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The cytokine antibody array experiments identified 23 proteins that differed significantly (p<0.05); of these, two proteins (TIMP1 and LIF) withstood the Bonferroni correction. In contrast, little difference was observed between CBMp and CPBp. At the diagnosis of ALL, changes in the soluble microenvironment are detectable in BM plasma. These changes probably participate in the pathogenesis and/or result from the changes in the cell composition.

Citovat

KOVAC, M; M VASKOVA; D PETRACKOVA; Vendula PELKOVÁ; E MEJSTRIKOVA; T KALINA; M ZALIOVA; J WEISER; J STARY a O HRUSAK. Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients. EUROPEAN CYTOKINE NETWORK. MONTROUGE: JOHN LIBBEY EUROTEXT LTD, 2014, roč. 25, č. 1, s. 8-13. ISSN 1148-5493. Dostupné z: https://doi.org/10.1684/ecn.2014.0348.

@article{1408695,
   author = {Kovac, M and Vaskova, M and Petrackova, D and Pelková, Vendula and Mejstrikova, E and Kalina, T and Zaliova, M and Weiser, J and Stary, J and Hrusak, O},
   article_location = {MONTROUGE},
   article_number = {1},
   doi = {https://doi.org/10.1684/ecn.2014.0348},
   keywords = {pediatric acute lymphoblastic leukemia; bone marrow plasma; cytokine antibody array},
   language = {eng},
   issn = {1148-5493},
   journal = {EUROPEAN CYTOKINE NETWORK},
   title = {Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients},
   volume = {25},
   year = {2014}
}

TY  - JOUR
ID  - 1408695
AU  - Kovac, M - Vaskova, M - Petrackova, D - Pelková, Vendula - Mejstrikova, E - Kalina, T - Zaliova, M - Weiser, J - Stary, J - Hrusak, O
PY  - 2014
TI  - Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients
JF  - EUROPEAN CYTOKINE NETWORK
VL  - 25
IS  - 1
SP  - 8-13
EP  - 8-13
PB  - JOHN LIBBEY EUROTEXT LTD
SN  - 11485493
KW  - pediatric acute lymphoblastic leukemia
KW  - bone marrow plasma
KW  - cytokine antibody array
N2  - Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of ALL cells. LBMp (leukemic bone marrow plasma) at the time of ALL diagnosis was compared to control plasma of bone marrow (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The cytokine antibody array enabled simultaneous detection of 79 proteins per sample. Candidate proteins exhibiting significantly different profiles were further analyzed and confirmed by ELISA. mRNAexpression of one of the candidate proteins (TIMP1) was studied using quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The cytokine antibody array experiments identified 23 proteins that differed significantly (p<0.05); of these, two proteins (TIMP1 and LIF) withstood the Bonferroni correction. In contrast, little difference was observed between CBMp and CPBp. At the diagnosis of ALL, changes in the soluble microenvironment are detectable in BM plasma. These changes probably participate in the pathogenesis and/or result from the changes in the cell composition.
ER  -

KOVAC, M; M VASKOVA; D PETRACKOVA; Vendula PELKOVÁ; E MEJSTRIKOVA; T KALINA; M ZALIOVA; J WEISER; J STARY a O HRUSAK. Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients. \textit{EUROPEAN CYTOKINE NETWORK}. MONTROUGE: JOHN LIBBEY EUROTEXT LTD, 2014, roč.~25, č.~1, s.~8-13. ISSN~1148-5493. Dostupné z: https://doi.org/10.1684/ecn.2014.0348.

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