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@proceedings{1409570,
author = {Šimara, Pavel and Tesařová, Lenka and Řeháková, Daniela and Farkaš, Šimon and Šalingová, Barbara and Kutálková, Kateřina and Vavrečková, Eva and Matula, Pavel and Matula, Petr and Krontorád Koutná, Irena},
booktitle = {SY-Stem symposium 2018},
keywords = {cellular reprogramming, endothelial differentiation},
language = {eng},
title = {Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells},
url = {https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/},
year = {2018}
}
TY - CONF
ID - 1409570
AU - Šimara, Pavel - Tesařová, Lenka - Řeháková, Daniela - Farkaš, Šimon - Šalingová, Barbara - Kutálková, Kateřina - Vavrečková, Eva - Matula, Pavel - Matula, Petr - Krontorád Koutná, Irena
PY - 2018
TI - Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells
KW - cellular reprogramming, endothelial differentiation
UR - https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/
N2 - Recent advances in human induced pluripotent stem cell (hiPSC) research have opened avenues to generate unlimited numbers of endothelial cells (ECs) from easily accessible cell sources, such as the peripheral blood. We reprogrammed peripheral blood mononuclear cells (PBMCs), human umbilical vein endothelial cells (HUVECs) and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. hiPSC-derived ECs were compared to HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts both phenotypically and functionally. An increased number of DNA double-strand breaks (γH2AX) upon reprogramming was observed. However, differentiation into ECs restored a normal number of γH2AX foci. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage.
ER -
ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA a Irena KRONTORÁD KOUTNÁ. Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells. In \textit{SY-Stem symposium 2018}. 2018.
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