Methods for Preparation of MS2 Phage-Like Particles and Their
Utilization as Process Control Viruses in RT-PCR and qRT-PCR
Detection of RNA Viruses From Food Matrices and Clinical
Specimens

J 2015

Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens

MIKEL, Pavel; Petra VAŠÍČKOVÁ a Petr KRÁLÍK

Základní údaje

Originální název

Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens

Autoři

MIKEL, Pavel; Petra VAŠÍČKOVÁ a Petr KRÁLÍK

Vydání

Food and Environmental Virology, New York, Springer, 2015, 1867-0334

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.338

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00108804

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MS2 phage-like particle; RNA virus; Process control virus; Detection; Quantification; Armored RNA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 12. 2019 11:49, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

RNA viruses are pathogenic agents of many serious infectious diseases affecting humans and animals. The detection of pathogenic RNA viruses is based on modern molecular methods, of which the most widely used methods are the reverse transcription polymerase chain reaction (RT-PCR) and the real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). All steps of RT-PCR and qRT-PCR should be strictly controlled to ensure the validity of obtained results. Falsenegative results may be caused not only by inhibition of RT or/and PCR steps but also by failure of the nucleic acid extraction step, particularly in the case of viral RNA extraction. The control of nucleic acid extraction generally involves the utilization of a non-pathogenic virus (process control virus) of similar structural properties to those of the target virus. Although in clinical samples the use of such process control virus is only recommended, in other kinds of settings such as food matrices its use is necessary. Currently, several different process control viruses are used for these purposes. Process control viruses can also be constructed artificially using technology for production of MS2 phage-like particles, which have many advantages in comparison with other used controls and are especially suited for controlling the detection and quantification of certain types of RNA viruses. The technology for production of MS2 phage-like particles is theoretically well established, uses the knowledge gained from the study of the familiar bacteriophage MS2 and utilizes many different approaches for the construction of the various process control viruses. Nevertheless, the practical use of MS2 phage-like particles in routine diagnostics is relatively uncommon. The current situation with regard to the use of MS2 phage-like particles as process control viruses in detection of RNA viruses and different methods of their construction, purification and use are summarized and discussed in this review.

Citovat

MIKEL, Pavel; Petra VAŠÍČKOVÁ a Petr KRÁLÍK. Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens. Food and Environmental Virology. New York: Springer, 2015, roč. 7, č. 2, s. 96-111. ISSN 1867-0334. Dostupné z: https://doi.org/10.1007/s12560-015-9188-2.

@article{1409951,
   author = {Mikel, Pavel and Vašíčková, Petra and Králík, Petr},
   article_location = {New York},
   article_number = {2},
   doi = {https://doi.org/10.1007/s12560-015-9188-2},
   keywords = {MS2 phage-like particle; RNA virus; Process control virus; Detection; Quantification; Armored RNA},
   language = {eng},
   issn = {1867-0334},
   journal = {Food and Environmental Virology},
   title = {Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens},
   url = {https://link.springer.com/content/pdf/10.1007%2Fs12560-015-9188-2.pdf},
   volume = {7},
   year = {2015}
}

TY  - JOUR
ID  - 1409951
AU  - Mikel, Pavel - Vašíčková, Petra - Králík, Petr
PY  - 2015
TI  - Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens
JF  - Food and Environmental Virology
VL  - 7
IS  - 2
SP  - 96-111
EP  - 96-111
PB  - Springer
SN  - 18670334
KW  - MS2 phage-like particle
KW  - RNA virus
KW  - Process control virus
KW  - Detection
KW  - Quantification
KW  - Armored RNA
UR  - https://link.springer.com/content/pdf/10.1007%2Fs12560-015-9188-2.pdf
L2  - https://link.springer.com/content/pdf/10.1007%2Fs12560-015-9188-2.pdf
N2  - RNA viruses are pathogenic agents of many serious infectious diseases affecting humans and animals. The detection of pathogenic RNA viruses is based on modern molecular methods, of which the most widely used methods are the reverse transcription polymerase chain reaction (RT-PCR) and the real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). All steps of RT-PCR and qRT-PCR should be strictly controlled to ensure the validity of obtained results. Falsenegative results may be caused not only by inhibition of RT or/and PCR steps but also by failure of the nucleic acid extraction step, particularly in the case of viral RNA extraction. The control of nucleic acid extraction generally involves the utilization of a non-pathogenic virus (process control virus) of similar structural properties to those of the target virus. Although in clinical samples the use of such process control virus is only recommended, in other kinds of settings such as food matrices its use is necessary. Currently, several different process control viruses are used for these purposes. Process control viruses can also be constructed artificially using technology for production of MS2 phage-like particles, which have many advantages in comparison with other used controls and are especially suited for controlling the detection and quantification of certain types of RNA viruses. The technology for production of MS2 phage-like particles is theoretically well established, uses the knowledge gained from the study of the familiar bacteriophage MS2 and utilizes many different approaches for the construction of the various process control viruses. Nevertheless, the practical use of MS2 phage-like particles in routine diagnostics is relatively uncommon. The current situation with regard to the use of MS2 phage-like particles as process control viruses in detection of RNA viruses and different methods of their construction, purification and use are summarized and discussed in this review.
ER  -

MIKEL, Pavel; Petra VAŠÍČKOVÁ a Petr KRÁLÍK. Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens. \textit{Food and Environmental Virology}. New York: Springer, 2015, roč.~7, č.~2, s.~96-111. ISSN~1867-0334. Dostupné z: https://doi.org/10.1007/s12560-015-9188-2.

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