Recent progress in nucleic acids isotachophoresis

J 2018

Recent progress in nucleic acids isotachophoresis

DATINSKÁ, Vladimíra; Ivona VORÁČOVÁ; Ulrich SCHLECHT; Jan BERKA; František FORET et al.

Základní údaje

Originální název

Recent progress in nucleic acids isotachophoresis

Autoři

DATINSKÁ, Vladimíra; Ivona VORÁČOVÁ; Ulrich SCHLECHT; Jan BERKA a František FORET

Vydání

Journal of Separation Science, Weinham, WILEY-VCH Verlag GmbH & Co. 2018, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.516

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00108808

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

isotachophoresis; nucleic acids; sample preparation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 2. 2020 11:49, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Progress achieved between 2014–2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.

Citovat

DATINSKÁ, Vladimíra; Ivona VORÁČOVÁ; Ulrich SCHLECHT; Jan BERKA a František FORET. Recent progress in nucleic acids isotachophoresis. Journal of Separation Science. Weinham: WILEY-VCH Verlag GmbH & Co., 2018, roč. 41, č. 1, s. 236-247. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201700878.

@article{1409998,
   author = {Datinská, Vladimíra and Voráčová, Ivona and Schlecht, Ulrich and Berka, Jan and Foret, František},
   article_location = {Weinham},
   article_number = {1},
   doi = {https://doi.org/10.1002/jssc.201700878},
   keywords = {isotachophoresis; nucleic acids; sample preparation},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Recent progress in nucleic acids isotachophoresis},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201700878},
   volume = {41},
   year = {2018}
}

TY  - JOUR
ID  - 1409998
AU  - Datinská, Vladimíra - Voráčová, Ivona - Schlecht, Ulrich - Berka, Jan - Foret, František
PY  - 2018
TI  - Recent progress in nucleic acids isotachophoresis
JF  - Journal of Separation Science
VL  - 41
IS  - 1
SP  - 236-247
EP  - 236-247
PB  - WILEY-VCH Verlag GmbH & Co.
SN  - 16159306
KW  - isotachophoresis
KW  - nucleic acids
KW  - sample preparation
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201700878
L2  - https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201700878
N2  - Progress achieved between 2014–2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.
ER  -

DATINSKÁ, Vladimíra; Ivona VORÁČOVÁ; Ulrich SCHLECHT; Jan BERKA a František FORET. Recent progress in nucleic acids isotachophoresis. \textit{Journal of Separation Science}. Weinham: WILEY-VCH Verlag GmbH \&{} Co., 2018, roč.~41, č.~1, s.~236-247. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201700878.

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