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@article{1410257,
author = {Nejdl, Lukas and Kudr, Jiri and Moulick, Amitava and Hegerova, Dagmar and RuttkayandNedecky, Branislav and Gumulec, Jaromír and Cihalova, Kristyna and Smerkova, Kristyna and Dostalova, Simona and Krizkova, Sona and Novotna, Marie and Kopel, Pavel and Adam, Vojtech},
article_location = {San Francisco},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0180798},
keywords = {Platinum nanoparticles; DNA},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Platinum nanoparticles induce damage to DNA and inhibit DNA replication},
volume = {12},
year = {2017}
}
TY - JOUR
ID - 1410257
AU - Nejdl, Lukas - Kudr, Jiri - Moulick, Amitava - Hegerova, Dagmar - Ruttkay-Nedecky, Branislav - Gumulec, Jaromír - Cihalova, Kristyna - Smerkova, Kristyna - Dostalova, Simona - Krizkova, Sona - Novotna, Marie - Kopel, Pavel - Adam, Vojtech
PY - 2017
TI - Platinum nanoparticles induce damage to DNA and inhibit DNA replication
JF - Plos one
VL - 12
IS - 7
SP - 1-19
EP - 1-19
PB - Public Library of Science
SN - 19326203
KW - Platinum nanoparticles
KW - DNA
N2 - Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage., on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.
ER -
NEJDL, Lukas, Jiri KUDR, Amitava MOULICK, Dagmar HEGEROVA, Branislav RUTTKAY-NEDECKY, Jaromír GUMULEC, Kristyna CIHALOVA, Kristyna SMERKOVA, Simona DOSTALOVA, Sona KRIZKOVA, Marie NOVOTNA, Pavel KOPEL a Vojtech ADAM. Platinum nanoparticles induce damage to DNA and inhibit DNA replication. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~7, s.~1-19. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0180798.
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